Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants

Seminario Huárriz, Amaia; Song, Li; Zulet González, Amaia; Nguyen, Henry T.; González García, Esther; Larrainzar Rodríguez, Estíbaliz

Publication:
Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants

dc.contributor.author	Seminario Huárriz, Amaia
dc.contributor.author	Song, Li	es_ES
dc.contributor.author	Zulet González, Amaia
dc.contributor.author	Nguyen, Henry T.	es_ES
dc.contributor.author	González García, Esther
dc.contributor.author	Larrainzar Rodríguez, Estíbaliz
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua, 2016/PI013	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 1287/2011	es
dc.date.accessioned	2018-09-04T06:43:52Z
dc.date.available	2018-09-04T06:43:52Z
dc.date.issued	2017
dc.description.abstract	Drought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought stress conditions. In the current work we analyze the effects of water deficit on the biosynthesis of AsA by measuring its content, in vivo biosynthesis and the expression level of genes in the Smirnoff-Wheeler pathway in one of the major legume crop, soybean (Glycine max L. Merr). Since the pathway has not been described in legumes, we first searched for the putative orthologous genes in the soybean genome. We observed a significant genetic redundancy, with multiple genes encoding each step in the pathway. Based on RNA-seq analysis, expression of the complete pathway was detected not only in leaves but also in root tissue. Putative paralogous genes presented differential expression patterns in response to drought, suggesting the existence of functional specialization mechanisms. We found a correlation between the levels of AsA and GalLDH biosynthetic rates in leaves of drought-stressed soybean plants. However, the levels of GalLDH transcripts did not show significant differences under water deficit conditions. Among the other known regulators of the pathway, only the expression of VTC1 genes correlated with the observed decline in AsA in leaves.	en
dc.description.sponsorship	This work was supported by the Spanish Ministry of Economy and Competitiveness (AGL 2011–23738 to EG and a “Juan de la Cierva” JCI-2012-13175 post-doctoral contract to EL) the Fundación Caja Navarra project FCN2016-7442 to EG and the project 2016/PI013 LEGUMINOSAS from the Government of Navarra to EL. AS received a Ph.D. fellowship from the Public University of Navarra (1287/2011).	en
dc.format.extent	10 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3389/fpls.2017.01042
dc.identifier.issn	1664-462X (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30445
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science, 8:1042	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-23738/ES/	en
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2017.01042
dc.rights	© 2017 Seminario, Song, Zulet, Nguyen, González and Larrainzar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	Ascorbic acid	en
dc.subject	Drought stress	en
dc.subject	Legumes	en
dc.subject	Smirnoff-Wheeler pathway	en
dc.subject	Soybean	en
dc.title	Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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