Molecular and insecticidal characterization of a novel cry-related protein from bacillus thuringiensis toxic against Myzus persicae

Palma Dovis, Leopoldo; Muñoz Labiano, Delia; Berry, Colin; Murillo Martínez, Jesús; Escudero, Iñigo de; Caballero Murillo, Primitivo

Molecular and insecticidal characterization of a novel cry-related protein from bacillus thuringiensis toxic against Myzus persicae

dc.contributor.author	Palma Dovis, Leopoldo
dc.contributor.author	Muñoz Labiano, Delia
dc.contributor.author	Berry, Colin
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.author	Escudero, Iñigo de
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2017-11-15T09:28:39Z
dc.date.available	2017-11-15T09:28:39Z
dc.date.issued	2014
dc.description.abstract	This study describes the insecticidal activity of a novel Bacillus thuringiensis Cry-related protein with a deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity to the 95-kDa-aphidicidal protein (sequence number 204) from patent US 8318900 and ~40% pairwise identity to the cancer cell killing Cry proteins (parasporins Cry41Ab1 and Cry41Aa1), respectively. This novel Cry-related protein contained the five conserved amino acid blocks and the three conserved domains commonly found in 3-domain Cry proteins. The protein exhibited toxic activity against the green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae) with the lowest mean lethal concentration (LC50 = 32.7 μg/mL) reported to date for a given Cry protein and this insect species, whereas it had no lethal toxicity against the Lepidoptera of the family Noctuidae Helicoverpa armigera (Hübner), Mamestra brassicae (L.), Spodoptera exigua (Hübner), S. frugiperda (J.E. Smith) and S. littoralis (Boisduval), at concentrations as high as ~3.5 μg/cm2. This novel Cry-related protein may become a promising environmentally friendly tool for the biological control of M. persicae and possibly also for other sap sucking insect pests.	en
dc.description.sponsorship	This research was supported by the Spanish Ministry of Science and Innovation (Grant Ref. AGL2009-13340-C02) and by the Universidad Pública de Navarra (contract awarded to L.P.).	en
dc.format.extent	13 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3390/toxins6113144
dc.identifier.issn	2072-6651
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/26160
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Toxins, 2014, 6(11), 3144-3156	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2009-13340-C02-02/ES/
dc.relation.publisherversion	https://dx.doi.org/10.3390/toxins6113144
dc.rights	© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Bacillus thuringiensis	en
dc.subject	δ-endotoxins	en
dc.subject	Cry-related protein	en
dc.subject	Parasporins	en
dc.subject	Green peach aphid	en
dc.subject	Myzus persicae	en
dc.title	Molecular and insecticidal characterization of a novel cry-related protein from bacillus thuringiensis toxic against Myzus persicae	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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