Activation of sweet pepper defense responses by novel and known biocontrol agents of the genus Bacillus against Botrytis cinerea and Verticillium dahliae

Poveda Arias, Jorge; Calvo, Javier; Barquero, Marcia; González Andrés, Fernando

Activation of sweet pepper defense responses by novel and known biocontrol agents of the genus Bacillus against Botrytis cinerea and Verticillium dahliae

dc.contributor.author	Poveda Arias, Jorge
dc.contributor.author	Calvo, Javier
dc.contributor.author	Barquero, Marcia
dc.contributor.author	González Andrés, Fernando
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2023-02-13T11:11:59Z
dc.date.available	2023-02-13T11:11:59Z
dc.date.issued	2022
dc.date.updated	2023-02-13T11:00:58Z
dc.description.abstract	The Fresno-Benavente Pepper (F-BP) Protected Geographical Indication (PGI) is a horticultural crop characterized by its great agronomic, economic and cultural importance in the region of Castilla y León (Spain). Field production is threatened by verticillium wilt caused by Verticillium dahliae and postharvest losses due to grey mould caused by Botrytis cinerea. Eight Bacillus spp. strains endophytically isolated from F-BP roots were used in the study. By conducting an in vitro antagonism study, we found that all Bacillus strains were effective against B. cinerea and five of them showed high antagonism against V. dahliae, with B. siamensis and B. proteolyticus strains being the most effective against both pathogens. Eight Bacillus strains were used for an infection test in F-BP fruits and plants to test their activity against both fungal pathogens. We report that Bacillus thuringiensis, B. siamensis and B. pumilus (SCFC 1–2) could control B. cinerea in pepper fruits through direct action and local activation of plant defences. In the case of V. dahliae root infection, plant roots inoculated with B. siamensis and B. proteolyticus were able to significantly decrease the occurrence of disease through direct action and local activation of jasmonic acid as a defence response. Therefore, we propose that B. siamensis could be used to control B. cinerea and V. dahliae in F-BP fruits and plants, respectively, through direct antagonism as well as the induction of local plant defence responses.	en
dc.description.sponsorship	Open Access funding provided by Universidad Pública de Navarra. This work was supported by the Instituto de Estudios Zamoranos “Florián de Ocampo” (IEZFO) as part of the XXVIII Call for Research Grants in 2020.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	image/tiff	en
dc.identifier.citation	Poveda, J., Calvo, J., Barquero, M., & González-Andrés, F. (2022). Activation of sweet pepper defense responses by novel and known biocontrol agents of the genus Bacillus against Botrytis cinerea and Verticillium dahliae. European Journal of Plant Pathology, 164(4), 507-524. https://doi.org/10.1007/s10658-022-02575-x	en
dc.identifier.doi	10.1007/s10658-022-02575-x
dc.identifier.issn	0929-1873
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44683
dc.language.iso	eng	en
dc.publisher	Springer	en
dc.relation.ispartof	European Journal of Plant Pathology (2022) 164:507–524	en
dc.relation.publisherversion	https://doi.org/10.1007/s10658-022-02575-x
dc.rights	© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Antagonism	en
dc.subject	Bacillus siamensis	en
dc.subject	Botrytis cinerea	en
dc.subject	Pepper	en
dc.subject	Plant defenses	en
dc.subject	Verticillium dahliae	en
dc.title	Activation of sweet pepper defense responses by novel and known biocontrol agents of the genus Bacillus against Botrytis cinerea and Verticillium dahliae	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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