Quinate-enhanced glyphosate toxicity is related to the accumulation of quinate derivatives

Zulet González, Ainhoa; Gil Monreal, Miriam; Gorzolka, Karin; Royuela Hernando, Mercedes; Zabalza Aznárez, Ana

Quinate-enhanced glyphosate toxicity is related to the accumulation of quinate derivatives

dc.contributor.author	Zulet González, Ainhoa
dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Gorzolka, Karin
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2024-09-18T17:52:14Z
dc.date.available	2024-09-18T17:52:14Z
dc.date.issued	2024
dc.date.updated	2024-09-18T17:42:40Z
dc.description.abstract	Glyphosate is the most widely used herbicide and works by inhibiting the enzyme 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) of the shikimate pathway, preventing the aromatic amino acid biosynthesis. When applied to plants, it provokes the accumulation of quinate, a metabolite synthesized through a side branch of the shikimate pathway. The joint application of glyphosate and quinate enhanced glyphosate efficacy on Amaranthus palmeri, requiring one-quarter of the recommended dose of glyphosate for complete control. Expression of the genes of the shikimate pathway and non-targeted GC-MS metabolic profiling were conducted to compare the physiological response after glyphosate, quinate or the combination of both. A perturbed gene expression of the shikimate pathway was detected after quinate applied alone, while no relevant changes in the metabolome were detected. The sub-lethal glyphosate treatment induced gene expression in the shikimate pathway, accumulation of the metabolites located upstream EPSPS and disturbances in the amino acid content. The exacerbation of the phytotoxicity in the lethal combined treatment was not related to any specific change in the expression level of the shikimate pathway. Metabolic profiling indicated that the accumulation of quinate and quinate derivatives detected after quinate applied alone was severely enhanced after the combined treatment of quinate and glyphosate.	en
dc.description.sponsorship	This work was funded by the Spanish Ministry of Economy and Competitiveness (AGL2016- 77531-R) and Spanish Ministry of Science and Innovation (2020 117723-RB-I00).
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Zulet-Gonzalez, A., Gil-Monreal, M., Gorzolka, K., Royuela, M., Zabalza, A. (2024) Quinate-enhanced glyphosate toxicity is related to the accumulation of quinate derivatives. Plant Stress, 12, 1-12. https://doi.org/10.1016/j.stress.2024.100496.
dc.identifier.doi	10.1016/j.stress.2024.100496
dc.identifier.issn	2667-064X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/51658
dc.language.iso	eng
dc.publisher	Elsevier
dc.relation.ispartof	Plant Stress 12, 2024, 100496
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2016-77531-R/
dc.relation.projectID	info:eu-repo/grantAgreement/AEI//2020 117723-RB-I00/
dc.relation.publisherversion	https://doi.org/10.1016/j.stress.2024.100496
dc.rights	© 2024 The Authors. This is an open access article under the CC BY-NC license.
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.subject	Amaranthus palmeri	en
dc.subject	Glyphosate	en
dc.subject	Metabolic profiling	en
dc.subject	Quinate	en
dc.subject	Shikimate pathway	en
dc.title	Quinate-enhanced glyphosate toxicity is related to the accumulation of quinate derivatives	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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