Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes

Cordero, P.; Campión, J.; Milagro Yoldi, F. I.; Marzo Pérez, Florencio; Martínez, J. A.

Publication:
Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes

dc.contributor.author	Cordero, P.
dc.contributor.author	Campión, J.
dc.contributor.author	Milagro Yoldi, F. I.
dc.contributor.author	Marzo Pérez, Florencio
dc.contributor.author	Martínez, J. A.
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.date.accessioned	2014-06-10T06:51:30Z
dc.date.available	2014-06-10T06:51:30Z
dc.date.issued	2008
dc.description.abstract	The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 +/- 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization of obesity and diabetes.	en
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1186/1476-511X-7-49
dc.identifier.issn	1476-511X
dc.identifier.other	717
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/10790
dc.language.iso	eng	en
dc.publisher	BioMed Central	en
dc.relation.ispartof	Lipids in Health and Disease, 2008, 7: 49	en
dc.relation.publisherversion	https://dx.doi.org/10.1186/1476-511X-7-49
dc.rights	© 2008 Cordero et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/2.0/
dc.subject	Tail vein injection	en
dc.subject	Plasmid delivery	en
dc.subject	Liver	en
dc.subject	Expression	en
dc.subject	Rats	en
dc.subject	UCP1	en
dc.subject	Manipulation	en
dc.subject	Mechanism	en
dc.subject	Obesity	en
dc.subject	Mice	en
dc.title	Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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