Effect of nutritional factors and copper on the regulation of laccase enzyme production in Pleurotus ostreatus
| dc.contributor.author | Durán Sequeda, Dinary Eloísa | |
| dc.contributor.author | Suspes, Daniela | |
| dc.contributor.author | Maestre, Estibenson | |
| dc.contributor.author | Alfaro Sánchez, Manuel | |
| dc.contributor.author | Pérez Garrido, María Gumersinda | |
| dc.contributor.author | Ramírez Nasto, Lucía | |
| dc.contributor.author | Pisabarro de Lucas, Gerardo | |
| dc.contributor.author | Sierra Ramírez, Rocío | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.date.accessioned | 2022-04-06T11:59:46Z | |
| dc.date.available | 2022-04-06T11:59:46Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | This research aimed to establish the relationship between carbon–nitrogen nutritional factors and copper sulfate on laccase activity (LA) by Pleurotus ostreatus. Culture media composition was tested to choose the nitrogen source. Yeast extract (YE) was selected as a better nitrogen source than ammonium sulfate. Then, the effect of glucose and YE concentrations on biomass production and LA as response variables was evaluated using central composite experimental designs with and without copper. The results showed that the best culture medium composition was glucose 45 gL−1 and YE 15 gL−1, simultaneously optimizing these two response variables. The fungal transcriptome was obtained in this medium with or without copper, and the differentially expressed genes were found. The main upregulated transcripts included three laccase genes (lacc2, lacc6, and lacc10) regulated by copper, whereas the principal downregulated transcripts included a copper transporter (ctr1) and a regulator of nitrogen metabolism (nmr1). These results suggest that Ctr1, which facilitates the entry of copper into the cell, is regulated by nutrient-sufficiency conditions. Once inside, copper induces transcription of laccase genes. This finding could explain why a 10–20-fold increase in LA occurs with copper compared to cultures without copper when using the optimal concentration of YE as nitrogen sources. | en |
| dc.description.sponsorship | This research was funded by project number RTI2018-099371-B-I00 (MCIU, AEI, FEDER/ UE) of the Spanish National Research Plan and the Public University of Navarre (UPNA) funds. D.D.-S. was supported by funds from the Gobernación del Departamento del Cesar by the Ministerio de Ciencia y Tecnología e Innovación de Colombia through 681 of the 2014 resolution. | en |
| dc.format.extent | 21 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.3390/jof8010007 | |
| dc.identifier.issn | 2309-608X | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/42633 | |
| dc.language.iso | eng | en |
| dc.publisher | MDPI | |
| dc.relation.ispartof | Journal of Fungi, 2022, 8 (1), 7 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099371-B-I00/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.3390/jof8010007 | |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Copper-transporter | en |
| dc.subject | Multi-copper oxidase | en |
| dc.subject | Nutrient-sufficient conditions | en |
| dc.subject | White-rot fungus | en |
| dc.title | Effect of nutritional factors and copper on the regulation of laccase enzyme production in Pleurotus ostreatus | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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