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Increased ascorbate biosynthesis does not improve nitrogen fixation nor alleviate the effect of drought stress in nodulated Medicago truncatula plants

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dc.contributor.authorCobos Porras, Inmaculada Libertad
dc.contributor.authorRubia Galiano, María Isabel
dc.contributor.authorHuertas, Raúl
dc.contributor.authorKum, David
dc.contributor.authorDalton, David A.
dc.contributor.authorUdvardi, Michael
dc.contributor.authorArrese-Igor Sánchez, César
dc.contributor.authorLarrainzar Rodríguez, Estíbaliz
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, PC112-113 LEGUSIes
dc.date.accessioned2021-10-13T07:52:41Z
dc.date.available2021-10-13T07:52:41Z
dc.date.issued2021
dc.description.abstractLegume plants are able to establish nitrogen-fixing symbiotic relations with Rhizobium bacteria. This symbiosis is, however, affected by a number of abiotic constraints, particularly drought. One of the consequences of drought stress is the overproduction of reactive oxygen (ROS) and nitrogen species (RNS), leading to cellular damage and, ultimately, cell death. Ascorbic acid (AsA), also known as vitamin C, is one of the antioxidant compounds that plants synthesize to counteract this oxidative damage. One promising strategy for the improvement of plant growth and symbiotic performance under drought stress is the overproduction of AsA via the overexpression of enzymes in the Smirnoff-Wheeler biosynthesis pathway. In the current work, we generated Medicago truncatula plants with increased AsA biosynthesis by overexpressing MtVTC2, a gene coding for GDP-L-galactose phosphorylase. We characterized the growth and physiological responses of symbiotic plants both under well-watered conditions and during a progressive water deficit. Results show that increased AsA availability did not provide an advantage in terms of plant growth or symbiotic performance either under well-watered conditions or in response to drought.en
dc.description.sponsorshipThis work had been funded by the Spanish Ministry of Science and Innovation-European Regional Development Fund (grant RTI2018-094623-B-C22) and the Government of Navarra (project PC112-113 LEGUSI). EL was a Ramón y Cajal fellow (RYC2018-023867-I) and LC-P and MR were Formación de Personal Investigador fellows from the Spanish Ministry of Economy and Competitiveness (BES-2015-074411 and BES-2012-059972, respectively).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.3389/fpls.2021.686075
dc.identifier.issn1664-462X
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/40741
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science June 2021, Volume 12, Article 686075en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094623-B-C22/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2018-023867-Ien
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BES-2015-074411/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BES-2012-059972/ES/en
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2021.686075
dc.rights© 2021 Cobos-Porras, Rubia, Huertas, Kum, Dalton, Udvardi, Arrese-Igor and Larrainzar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAscorbic aciden
dc.subjectLegumeen
dc.subjectSymbiosisen
dc.subjectWater deficiten
dc.subjectAntioxidanten
dc.titleIncreased ascorbate biosynthesis does not improve nitrogen fixation nor alleviate the effect of drought stress in nodulated Medicago truncatula plantsen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dspace.entity.typePublication
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