Publication: The toxic guardians: multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids
| dc.contributor.author | Bardají Goikoetxea, Leire | |
| dc.contributor.author | Añorga García, Maite | |
| dc.contributor.author | Echeverría Ancín, Myriam | |
| dc.contributor.author | Ramos, Cayo | |
| dc.contributor.author | Murillo Martínez, Jesús | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.date.accessioned | 2019-09-25T10:39:34Z | |
| dc.date.available | 2019-09-25T10:39:34Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Background: Pseudomonas syringae is a y-proteobacterium causing economically relevant diseases in practically all cultivated plants. Most isolates of this pathogen contain native plasmids collectively carrying many pathogenicity and virulence genes. However, P. syringae is generally an opportunistic pathogen primarily inhabiting environmental reservoirs, which could exert a low selective pressure for virulence plasmids. Additionally, these plasmids usually contain a large proportion of repeated sequences, which could compromise plasmid integrity. Therefore, the identification of plasmid stability determinants and mechanisms to preserve virulence genes is essential to understand the evolution of this pathogen and its adaptability to agroecosystems. Results: The three virulence plasmids of P. syringae pv. savastanoi NCPPB 3335 contain from one to seven functional stability determinants, including three highly active toxin-antitoxin systems (TA) in both pPsv48A and pPsv48C. The TA systems reduced loss frequency of pPsv48A by two orders of magnitude, whereas one of the two replicons of pPsv48C likely confers stable inheritance by itself. Notably, inactivation of the TA systems from pPsv48C exposed the plasmid to high-frequency deletions promoted by mobile genetic elements. Thus, recombination between two copies of MITEPsy2 caused the deletion of an 8.3 kb fragment, with a frequency of 3.8 ± 0.3 x 10-3. Likewise, one-ended transposition of IS801 generated plasmids containing deletions of variable size, with a frequency of 5.5 ± 2.1 x 1 0- 4, of which 80% had lost virulence gene idi. These deletion derivatives were stably maintained in the population by replication mediated by repJ, which is adjacent to IS801. IS801 also promoted deletions in plasmid pPsv48A, either by recombination or one-ended transposition. In all cases, functional TA systems contributed significantly to reduce the occurrence of plasmid deletions in vivo. Conclusions: Virulence plasmids from P. syringae harbour a diverse array of stability determinants with a variable contribution to plasmid persistence. Importantly, we showed that multiple plasmid-borne TA systems have a prominent role in preserving plasmid integrity and ensuring the maintenance of virulence genes in free-living conditions. This strategy is likely widespread amongst native plasmids of P. syringae and other bacteria. | en |
| dc.description.sponsorship | This work was funded by the Spanish Plan Nacional I + D + I grants AGL2014–53242-C2–1-R, AGL2014–53242-C2–2-R, AGL2017-82492-C2-1- R, and AGL2017-82492-C2-2-R from the Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER). | en |
| dc.format.extent | 24 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1186/s13100-019-0149-4 | |
| dc.identifier.issn | 1759-8753 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/34934 | |
| dc.language.iso | eng | en |
| dc.publisher | BMC | en |
| dc.relation.ispartof | Mobile DNA, (2019), 10:7 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2014-53242-C2-2-R/ES/ | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R/ES/ | en |
| dc.relation.publisherversion | https://doi.org/10.1186/s13100-019-0149-4 | |
| dc.rights | © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
| dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Postsegregational killing | en |
| dc.subject | Native plasmid evolution | en |
| dc.subject | IS801 | en |
| dc.subject | Replicative transposition | en |
| dc.subject | IS91 family | en |
| dc.subject | Olive knot disease | en |
| dc.subject | Pathogenicity | en |
| dc.subject | MITEs | en |
| dc.subject | One-ended transposition | en |
| dc.subject | Pseudomonas savastanoi | en |
| dc.title | The toxic guardians: multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids | en |
| dc.type | info:eu-repo/semantics/article | en |
| dc.type | Artículo / Artikulua | es |
| dc.type.version | info:eu-repo/semantics/publishedVersion | en |
| dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
| dspace.entity.type | Publication | |
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