Bacmid expression of granulovirus enhancin En3 accumulates in cell soluble fraction to potentiate nucleopolyhedrovirus infection
| dc.contributor.author | Ricarte Bermejo, Adriana | |
| dc.contributor.author | Simón de Goñi, Oihane | |
| dc.contributor.author | Fernández González, Ana Beatriz | |
| dc.contributor.author | Williams, Trevor | |
| dc.contributor.author | Caballero Murillo, Primitivo | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.date.accessioned | 2021-11-11T09:46:54Z | |
| dc.date.available | 2021-11-11T09:46:54Z | |
| dc.date.issued | 2021 | |
| dc.description | Incluye material complementario | es_ES |
| dc.description.abstract | Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In nucleopolyhedroviruses (NPVs), in contrast, they are present in the envelope of the occlusion-derived virions (ODV). In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of enhancin in the OBs or ODVs. En3 was successfully produced but did not localize to the OBs or the ODVs and accumulated in the soluble fraction of infected cells. As a result, increased OB pathogenicity was observed when OBs were administered in mixtures with the soluble fraction of infected cells. The mixture of OBs and the soluble fraction of Sf9 cells infected with BacPhEn3 recombinant virus was ~3- and ~4.7-fold more pathogenic than BacPh control OBs in the second and fourth instars of Spodoptera exigua, respectively. In contrast, when purified, recombinant BacPhEn3 OBs were as pathogenic as control BacPh OBs. The expression of En3 in the soluble fraction of insect cells may find applications in the development of virus-based insecticides with increased efficacy. | en |
| dc.description.sponsorship | This study received financial support from the Spanish Ministry project AGL2017-83498-C2-1-R. Bioinsectis SL funded the activities of ABF. | es_ES |
| dc.format.extent | 17 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/zip | en |
| dc.identifier.doi | 10.3390/v13071233 | |
| dc.identifier.issn | 1999-4915 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/41032 | |
| dc.language.iso | eng | en |
| dc.publisher | MDPI | en |
| dc.relation.ispartof | Viruses 2021, 13, 1233 | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83498-C2-1-R/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.3390/v13071233 | |
| dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Alphabaculovirus | en |
| dc.subject | Betabaculovirus | en |
| dc.subject | Enhancins | en |
| dc.subject | AcMNPV recombinant | en |
| dc.subject | Spodoptera exigua | en |
| dc.subject | Potentiation of pathogenicity | en |
| dc.title | Bacmid expression of granulovirus enhancin En3 accumulates in cell soluble fraction to potentiate nucleopolyhedrovirus infection | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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