Comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus Pleurotus ostreatus

Alfaro Sánchez, Manuel; Castanera Andrés, Raúl; Lavín Trueba, José Luis; Oguiza Tomé, José Antonio; Ramírez Nasto, Lucía; Pisabarro de Lucas, Gerardo

Publication:
Comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus Pleurotus ostreatus

dc.contributor.author	Alfaro Sánchez, Manuel
dc.contributor.author	Castanera Andrés, Raúl
dc.contributor.author	Lavín Trueba, José Luis
dc.contributor.author	Oguiza Tomé, José Antonio
dc.contributor.author	Ramírez Nasto, Lucía
dc.contributor.author	Pisabarro de Lucas, Gerardo
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2020-09-22T07:53:19Z
dc.date.available	2020-09-22T07:53:19Z
dc.date.issued	2016
dc.description.abstract	Fungi interact with their environment by secreting proteins to obtain nutrients, elicit responses and modify their surroundings. Because the set of proteins secreted by a fungus is related to its lifestyle, it should be possible to use it as a tool to predict fungal lifestyle. To test this hypothesis, we bioinformatically identified 538 and 554 secretable proteins in the monokaryotic strains PC9 and PC15 of the white rot basidiomycete Pleurotus ostreatus. Functional annotation revealed unknown functions (37.2%), glycosyl hydrolases (26.5%) and redox enzymes (11.5%) as the main groups in the two strains. When these results were combined with RNA‐seq analyses, we found that the relative importance of each group was different in different strains and culture conditions and the relevance of the unknown function proteins was enhanced. Only a few genes were actively expressed in a given culture condition in expanded multigene families, suggesting that family expansi on could increase adaptive opportunities rather than activity under a specific culture condition. Finally, we used the set of P. ostreatus secreted proteins as a query to search their counterparts in other fungal genomes and found that the secretome profiles cluster the tested basidiomycetes into lifestyle rather than phylogenetic groups.	en
dc.description.sponsorship	This work was supported by funds from the project AGL2011‐30495 of the Spanish National Research Plan, by additional institutional support from the Public University of Navarre, and by the Office of Science of the U.S. Department of Energy under Contract No. DE‐AC02‐05CH11231 (work conducted by the U.S. Department of Energy Joint Genome Institute). JLL is supported by the Basque Country Government (Etortek Research Programs 2011/2014) and from the Innovation Technology Dept. of Bizkaia.	en
dc.format.extent	17 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1111/1462-2920.13360
dc.identifier.issn	1462-2920
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38220
dc.language.iso	eng	en
dc.publisher	Wiley	en
dc.publisher	Society for Applied Microbiology	en
dc.relation.ispartof	Environmental Microbiology, 2016, 18(12), 4710-4726	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30495/ES/	en
dc.relation.publisherversion	https://doi.org/10.1111/1462-2920.13360
dc.rights	© 2016 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Fungi	en
dc.subject	Pleurotus ostreatus	en
dc.subject	Protein secretions	en
dc.title	Comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus Pleurotus ostreatus	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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