Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides

Barco Antoñanzas, María; Gil Monreal, Miriam; Eceiza, Mikel Vicente; Royuela Hernando, Mercedes; Zabalza Aznárez, Ana

Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides

dc.contributor.author	Barco Antoñanzas, María
dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Eceiza, Mikel Vicente
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA 2010	es
dc.date.accessioned	2022-02-18T11:51:54Z
dc.date.available	2022-02-18T11:51:54Z
dc.date.issued	2022
dc.description	Datos de investigación alojados en: https://doi.org/10.5281/zenodo.15875028
dc.description.abstract	The objective of this work was to characterize the resistance mechanisms and the primary metabolism of a multiple resistant (MR) population of Amaranthus palmeri to glyphosate and to the acetolactate synthase (ALS) inhibitor pyrithiobac. All MR plants analysed were glyphosate-resistant due to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene amplification. Resistance to pyrithiobac was more variable among individuals and was related to point mutations at five positions in the ALS gene sequence: A122, A205, W574, S653 and G654. All MR plants were heterozygous for W574, the most abundant mutation. In nontreated plants, the presence of mutations did not affect ALS functionality, and plants with the W574L mutation showed the highest ALS resistance level to pyrithiobac. The accumulation of the transcripts corresponding to several genes of the aromatic amino acid (AAA) and branched-chain amino acid (BCAA) pathways detected in nontreated MR plants indicated additional effects of EPSPS gene amplification and ALS mutations. The physiological performance of the MR population after treatment with glyphosate and/or pyrithiobac was compared with that of a sensitive (S) population. The increase induced in total soluble sugars, AAA or BCAA content by both herbicides was higher in the S population than in the MR population. Physiological effects were not exacerbated after the mixture of both herbicides in S or in MR populations. This study provides new insights into the physiology of a multiple resistant A. palmeri, which could be very useful for achieving effective management of this weed.	en
dc.description.sponsorship	This work was funded by the Spanish Ministry of Economy and Competitiveness (AGL2016-77531-R), Spain and by the Universidad Pública de Navarra, Spain (Project UPNA20-6138). M.B.-A. received funding from fellowship through Universidad Pública de Navarra. M.V.E. is the holder of a predoctoral fellowship of the Basque Government.	en
dc.description.sponsorship	This work was funded by the Spanish Ministry of Economy andCompetitiveness (AGL2016-77531-R) and Spanish Ministry of Scienceand Innovation (2020 117723-RB-100). Maria Barco-Antoñanzas.received funding from PhD fellowship and mobility grant throughUniversidad Pública de Navarra. Mikel V. Eceiza is the holder of apredoctoral fellowship of the Basque Government. We thank GustavoGarijo and Clara Jimenez for technical assistance. Open access fundingprovided by Universidad Pública de Navarra.
dc.format.extent	16 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.plantsci.2022.111212
dc.identifier.issn	0168-9452
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/42274
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Plant Science 318 (2022) 111212	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI//AGL2016-77531-R/ES/
dc.relation.publisherversion	https://doi.org/10.1016/j.plantsci.2022.111212
dc.rights	© 2022 The Authors. This is an open access article under the CC BY-NC-ND license	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Pyrithiobac	en
dc.subject	Glyphosate	en
dc.subject	Acetolactate synthase	en
dc.subject	Palmer amaranth	en
dc.subject	5-enolpyruvylshikimate-3-phosphate synthase	en
dc.subject	Target site resistance	en
dc.title	Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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