Identification of new antifungal metabolites produced by the yeast Metschnikowia pulcherrima involved in the biocontrol of postharvest plant pathogenic fungi

Fernández San Millán, Alicia; Gamir, Jordi; Farrán Blanch, Inmaculada; Larraya Reta, Luis María; Veramendi Charola, Jon

Publication:
Identification of new antifungal metabolites produced by the yeast Metschnikowia pulcherrima involved in the biocontrol of postharvest plant pathogenic fungi

dc.contributor.author	Fernández San Millán, Alicia
dc.contributor.author	Gamir, Jordi
dc.contributor.author	Farrán Blanch, Inmaculada
dc.contributor.author	Larraya Reta, Luis María
dc.contributor.author	Veramendi Charola, Jon
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2023-03-23T11:11:36Z
dc.date.available	2023-03-23T11:11:36Z
dc.date.issued	2022
dc.date.updated	2023-03-23T09:29:37Z
dc.description.abstract	Several strains of the yeast Metschnikowia pulcherrima exhibit strong antagonistic activity against postharvest pathogens and may have broad biotechnological potential as biocontrol agents. However, the nature and interplay of the mechanisms contributing to this antifungal activity are still largely unknown. This study characterizes the antifungal compounds present in the exometabolome of two yeast strains that previously showed an efficient inhibition of Botrytis cinerea infection. We show that a yeast-fungus co-culture assay is a good system to examine the antagonistic interaction and elucidate the nature of the produced yeast metabolites. As a result, our UPLC-MS/MS analysis identified a total of 35 differentially secreted metabolites, potentially involved in the biocontrol of gray mold. Subsequent in vitro analysis and in vivo tomato, grape and apple fruit protection assays with such metabolites allowed us to identify several new antifungal compounds, with 3-amino-5-methylhexanoic acid, biphenyl-2,3-diol and sinapaldehyde being the most active (with up to 90–100% reduction in the infection of tomato and apple with B. cinerea). In addition, the first two metabolites protected tomatoes against Alternaria alternata infection. It was observed that these metabolites negatively affected the cell membrane integrity and mycelial morphology of B. cinerea and increased the intracellular level of ROS. Furthermore, other unexpected metabolites with interesting biotechnological applications were identified for the first time as being secreted by yeast cells, such as piperideine and protoemetine (alkaloids), p-coumaroyl quinic acid (phenylpropanoid), β-rhodomycin (antibiotic), hexadecanedioic acid (long chain fatty acid) or taurocholic acid (bile acid). This fact highlights that the antifungal activity of M. pulcherrima may result from synergistic action of several active molecules.	en
dc.description.sponsorship	This work was financed by the Departamento de Desarrollo Económico y Empresarial from the Gobierno de Navarra (Spain): grants 0011-1365-2021-000079 and 0011-1411-2019-000009. Open Access funding provided by Universidad Pública de Navarra.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/msword	en
dc.identifier.citation	Fernández-San-Millan, A., Gamir, J., Farran, I., Larraya, L., Veramendi, J. (2022) Identification of new antifungal metabolites produced by the yeast Metschnikowia pulcherrima involved in the biocontrol of postharvest plant pathogenic fungi. Postharvest Biology and Technology, 192. https://doi.org/10.1016/j.postharvbio.2022.111995.	en
dc.identifier.doi	10.1016/j.postharvbio.2022.111995
dc.identifier.issn	0925-5214
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44951
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Postharvest Biology and Technology 192 (2022) 111995	en
dc.relation.publisherversion	https://doi.org/10.1016/j.postharvbio.2022.111995
dc.rights	© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Biocontrol	en
dc.subject	Botrytis	en
dc.subject	Exometabolome	en
dc.subject	Metabolomic	en
dc.subject	Metschnikowia	en
dc.subject	Postharvest	en
dc.title	Identification of new antifungal metabolites produced by the yeast Metschnikowia pulcherrima involved in the biocontrol of postharvest plant pathogenic fungi	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
relation.isAuthorOfPublication	9c84c660-8e77-4487-bd7a-07ad058b0425
relation.isAuthorOfPublication	6442ab25-7b61-4611-a7b8-d237c8cf5d82
relation.isAuthorOfPublication	62d7811e-d4cb-42e6-90ee-8e9dc4f8a95c
relation.isAuthorOfPublication	a901e048-1a47-49df-a6f4-c2012c88c29e
relation.isAuthorOfPublication.latestForDiscovery	9c84c660-8e77-4487-bd7a-07ad058b0425