Mass spectrometry-based proteomic profiling of thrombotic material obtained by endovascular thrombectomy in patients with ischemic stroke

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dc.contributor.authorMuñoz, Roberto
dc.contributor.authorSantamaría Martínez, Enrique
dc.contributor.authorRubio, Idoya
dc.contributor.authorAusín, Karina
dc.contributor.authorLabarga Gutiérrez, Alberto
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, PC025es
dc.date.accessioned2020-12-02T10:17:52Z
dc.date.available2020-12-02T10:17:52Z
dc.date.issued2018
dc.description.abstractThrombotic material retrieved from acute ischemic stroke (AIS) patients represents a valuable source of biological information. In this study, we have developed a clinical proteomics workflow to characterize the protein cargo of thrombi derived from AIS patients. To analyze the thrombus proteome in a large-scale format, we developed a workflow that combines the isolation of thrombus by endovascular thrombectomy and peptide chromatographic fractionation coupled to mass-spectrometry. Using this workflow, we have characterized a specific proteomic expression profile derived from four AIS patients included in this study. Around 1600 protein species were unambiguously identified in the analyzed material. Functional bioinformatics analyses were performed, emphasizing a clustering of proteins with immunological functions as well as cardiopathy-related proteins with blood-cell dependent functions and peripheral vascular processes. In addition, we established a reference proteomic fingerprint of 341 proteins commonly detected in all patients. Protein interactome network of this subproteome revealed protein clusters involved in the interaction of fibronectin with 14-3-3 proteins, TGFβ signaling, and TCP complex network. Taken together, our data contributes to the repertoire of the human thrombus proteome, serving as a reference library to increase our knowledge about the molecular basis of thrombus derived from AIS patients, paving the way toward the establishment of a quantitative approach necessary to detect and characterize potential novel biomarkers in the stroke field.en
dc.description.sponsorshipThis work was supported by Navarre Government Funding (Industry and Health department) through ADITECH (PT030) and Department of Economic Development from Government of Navarra (Ref. PC025), and Obra Social la Caixa to ES. KA are supported by PEJ-2014-A-61949 (MINECO). The Proteomics Unit of Navarrabiomed is a member of Proteored, PRB3-ISCIII, and is supported by grant PT17/0019/009 to JF, of the PE I+D+I 2013–2016 funded by ISCIII and FEDERen
dc.format.extent17 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.3390/ijms19020498
dc.identifier.issn1422-0067
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/38817
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofInternational Journal of Molecular Sciences, 2018, 19(2): 498en
dc.relation.publisherversionhttps://doi.org/10.3390/ijms19020498
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access_x000D_ article distributed under the terms and conditions of the Creative Commons Attribution_x000D_ (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectIschemic strokeen
dc.subjectProteomicsen
dc.subjectBiomarkersen
dc.subjectEndovascular therapyen
dc.subjectThrombusen
dc.titleMass spectrometry-based proteomic profiling of thrombotic material obtained by endovascular thrombectomy in patients with ischemic strokeen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicationabacfd17-2b93-4d99-bae2-52053d57401e
relation.isAuthorOfPublication1d1bfb61-da54-4d1b-a8b3-e669a4b98016
relation.isAuthorOfPublication.latestForDiscovery1d1bfb61-da54-4d1b-a8b3-e669a4b98016

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