Host range determinants of Pseudomonas savastanoi pathovars of woody hosts revealed by comparative genomics and cross-pathogenicity tests

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dc.contributor.authorMoreno Pérez, Alba
dc.contributor.authorPintado, Adrián
dc.contributor.authorMurillo Martínez, Jesús
dc.contributor.authorCaballo Ponce, Eloy
dc.contributor.authorTegli, Stefania
dc.contributor.authorMoretti, Chiaraluce
dc.contributor.authorRodríguez Palenzuela, Pablo
dc.contributor.authorRamos, Cayo
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.date.accessioned2020-08-03T08:43:49Z
dc.date.available2020-08-03T08:43:49Z
dc.date.issued2020
dc.description.abstractThe study of host range determinants within the Pseudomonas syringae complex is gaining renewed attention due to its widespread distribution in non-agricultural environments, evidence of large variability in intra-pathovar host range, and the emergence of new epidemic diseases. This requires the establishment of appropriate model pathosystems facilitating integration of phenotypic, genomic and evolutionary data. Pseudomonas savastanoi pv. savastanoi is a model pathogen of the olive tree, and here we report a closed genome of strain NCPPB 3335, plus draft genome sequences of three strains isolated from oleander (pv. nerii), ash (pv. fraxini) and broom plants (pv. retacarpa). We then conducted a comparative genomic analysis of these four new genomes plus 16 publicly available genomes, representing 20 strains of these four P. savastanoi pathovars of woody hosts. Despite overlapping host ranges, cross-pathogenicity tests using four plant hosts clearly separated these pathovars and lead to pathovar reassignment of two strains. Critically, these functional assays were pivotal to reconcile phylogeny with host range and to define pathovar-specific genes repertoires. We report a pan-genome of 7,953 ortholog gene families and a total of 45 type III secretion system effector genes, including 24 core genes, four genes exclusive of pv. retacarpa and several genes encoding pathovar-specific truncations. Noticeably, the four pathovars corresponded with well-defined genetic lineages, with core genome phylogeny and hierarchical clustering of effector genes closely correlating with pathogenic specialization. Knot-inducing pathovars encode genes absent in the canker-inducing pv. fraxini, such as those related to indole acetic acid, cytokinins, rhizobitoxine, and a bacteriophytochrome. Other pathovar-exclusive genes encode type I, type II, type IV, and type VI secretion system proteins, the phytotoxine phevamine A, a siderophore, c-di-GMP-related proteins, methyl chemotaxis proteins, and a broad collection of transcriptional regulators and transporters of eight different superfamilies. Our combination of pathogenicity analyses and genomics tools allowed us to correctly assign strains to pathovars and to propose a repertoire of host range-related genes in the P. syringae complex.en
dc.description.sponsorshipAM-P, AP, CR, and JM were supported by grants FPI/BES-2015-074847, FPU14/05551, AGL2017-82492-C2-1-R and AGL2017-82492-C2-2-R, respectively, from Ministerio de Ciencia, Innovacion y Universidades (Spain), cofinanced by the Fondo Europeo de Desarrollo Regional (FEDER); CM was supported by DSA3 research funds “Fondo di base” (Italy).en
dc.format.extent19 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.3389/fpls.2020.00973
dc.identifier.issn1664-462X (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/37612
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science 11:973en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R/ES/
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2020.00973
dc.rights© 2020 Moreno-Perez, Pintado, Murillo, Caballo-Ponce, Tegli, Moretti, Rodrıguez-Palenzuela and Ramos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPseudomonas syringaeen
dc.subjectPseudomonas savastanoi pathovarsen
dc.subjectWoody hostsen
dc.subjectHost rangeen
dc.subjectVirulence factorsen
dc.subjectComparative genomicsen
dc.subjectType III effectorsen
dc.subjectPhytotoxinsen
dc.titleHost range determinants of Pseudomonas savastanoi pathovars of woody hosts revealed by comparative genomics and cross-pathogenicity testsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery3f350c70-2e68-4b9b-95d6-26e9cc1540a0

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