Expression of a peroral infection factor determines pathogenicity and population structure in an insect virus

Simón de Goñi, Oihane; Williams, Trevor; Cerutti, Martine; Caballero Murillo, Primitivo; López Ferber, Miguel

Expression of a peroral infection factor determines pathogenicity and population structure in an insect virus

dc.contributor.author	Simón de Goñi, Oihane
dc.contributor.author	Williams, Trevor
dc.contributor.author	Cerutti, Martine
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.author	López Ferber, Miguel
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-09-06T12:20:26Z
dc.date.available	2018-09-06T12:20:26Z
dc.date.issued	2013
dc.description.abstract	A Nicaraguan isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus is being studied as a possible biological insecticide. This virus exists as a mixture of complete and deletion genotypes; the latter depend on the former for the production of an essential per os transmission factor (pif1) in coinfected cells. We hypothesized that the virus population was structured to account for the prevalence of pif1 defector genotypes, so that increasing the abundance of pif1 produced by a cooperator genotype in infected cells would favor an increased prevalence of the defector genotype. We tested this hypothesis using recombinant viruses with pif1 expression reprogrammed at its native locus using two exogenous promoters (egt, p10) in the pif2/pif1 intergenic region. Reprogrammed viruses killed their hosts markedly faster than the wild-type and rescue viruses, possibly due to an earlier onset of systemic infection. Group success (transmission) depended on expression of pif1, but overexpression was prejudicial to group-specific transmissibility, both in terms of reduced pathogenicity and reduced production of virus progeny from each infected insect. The presence of pif1-overproducing genotypes in the population was predicted to favor a shift in the prevalence of defector genotypes lacking pif1-expressing capabilities, to compensate for the modification in pif1 availability at the population level. As a result, defectors increased the overall pathogenicity of the virus population by diluting pif1 produced by overexpressing genotypes. These results offer a new and unexpected perspective on cooperative behavior between viral genomes in response to the abundance of an essential public good that is detrimental in excess.	en
dc.description.sponsorship	This study received financial support from the Spanish Ministry for Science and Technology projects AGL2005-07909-CO3-01 and AGL2008-05456-CO3- 01/AGR. O.S. received a Jose´ Castillejo grant for postdoctoral mobility.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0078834
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30566
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 8(11): e78834	en
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0078834
dc.rights	© 2013 Simón et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	PIF factors	en
dc.subject	Spodoptera frugiperda multiple nucleopolyhedrovirus	en
dc.subject	Biological insecticides	en
dc.title	Expression of a peroral infection factor determines pathogenicity and population structure in an insect virus	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	5b9012ab-c911-4ef7-9a92-0041bc07bc6f
relation.isAuthorOfPublication	0b5a3470-9d73-4ac7-a923-c4f6079df358
relation.isAuthorOfPublication	ecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery	5b9012ab-c911-4ef7-9a92-0041bc07bc6f