Publication:
Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion

Simple item page
dc.contributor.authorMartínez, Diego
dc.contributor.authorChallacombe, Jean
dc.contributor.authorMorgenstern, Ingo
dc.contributor.authorHibbett, David
dc.contributor.authorSchmoll, Monika
dc.contributor.authorKubicek, Christian P.
dc.contributor.authorFerreira, Patricia
dc.contributor.authorPisabarro de Lucas, Gerardo
dc.contributor.authorLavín Trueba, José Luis
dc.contributor.authorOguiza Tomé, José Antonio
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.date.accessioned2018-12-28T09:14:03Z
dc.date.available2018-12-28T09:14:03Z
dc.date.issued2009
dc.description.abstractBrown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative β -1–4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also upregulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H2O2. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H2O2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.en
dc.description.sponsorshipThis work was supported by the U.S. Department of Energy’s Office of Science, Biological and Environmental Research Program, and University of California, Lawrence Berkeley National Laboratory Contract DE-AC02–05CH11231; Lawrence Livermore National Laboratory Contract DE-AC52–07NA27344; Los Alamos National Laboratory Contract DE-AC02–06NA25396; University of Wisconsin Grant DE-FG02–87ER13712; Forest Products Laboratory, U.S. Department of Agriculture, Cooperative State Research, Education, and Extension Services Grant 2007–35504-18257; National Institutes of Health Grant GM060201 (to University of New Mexico); Centro de Investigaciones Biológicas (Madrid) EUproject NMP2–2006-026456; Ministry of Education Czech Republic Grant LC06066.en
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1073/pnas.0809575106
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/31882
dc.language.isoengen
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS, February 10, 2009 106 (6) 1954-1959en
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.0809575106
dc.rights© 2009 by The National Academy of Sciences of the USAen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.subjectPostia placentaen
dc.subjectCelluloseen
dc.subjectFentonen
dc.subjectLigninen
dc.subjectCellulaseen
dc.subjectBrown-roten
dc.titleGenome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversionen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
relation.isAuthorOfPublication54daf1c4-f273-4ade-a90e-7df28e761ca7
relation.isAuthorOfPublication01fafca5-7f50-4671-bcd0-a0a93a0d7c98
relation.isAuthorOfPublication0a109d77-5b6c-41ee-b859-c75cc8b012ed
relation.isAuthorOfPublication.latestForDiscovery54daf1c4-f273-4ade-a90e-7df28e761ca7

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
16_Martinez_GenomeTranscriptome.pdf
Size:
1.09 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
16_MatCompl.zip
Size:
1.11 MB
Format:
ZIP
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista DPA - NES Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak