Induction of radiata pine somatic embryogenesis at high temperatures provokes a long-term decrease in DNA methylation/hydroxymethylation and differential expression of stress-related genes
| dc.contributor.author | Castander Olarieta, Ander | |
| dc.contributor.author | Pereira, Catia | |
| dc.contributor.author | Sales, Ester | |
| dc.contributor.author | Meijón, Mónica | |
| dc.contributor.author | Arrillaga, Isabel | |
| dc.contributor.author | Goicoa Mangado, Tomás | |
| dc.contributor.author | Ugarte Martínez, María Dolores | |
| dc.contributor.author | Cañal, María Jesús | |
| dc.contributor.author | Moncaleán, Paloma | |
| dc.contributor.author | Montalbán, Itziar A. | |
| dc.contributor.department | Estadística, Informática y Matemáticas | es_ES |
| dc.contributor.department | Estatistika, Informatika eta Matematika | eu |
| dc.contributor.department | Institute for Advanced Materials and Mathematics - INAMAT2 | en |
| dc.date.accessioned | 2021-06-16T08:48:55Z | |
| dc.date.available | 2021-06-16T08:48:55Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Based on the hypothesis that embryo development is a crucial stage for the formation of stable epigenetic marks that could modulate the behaviour of the resulting plants, in this study, radiata pine somatic embryogenesis was induced at high temperatures (23◦ C, eight weeks, control; 40◦ C, 4 h; 60◦ C, 5 min) and the global methylation and hydroxymethylation levels of emerging embryonal masses and somatic plants were analysed using LC-ESI-MS/ MS-MRM. In this context, the expression pattern of six genes previously described as stress-mediators was studied throughout the embryogenic process until plant level to assess whether the observed epigenetic changes could have provoked a sustained alteration of the transcriptome. Results indicated that the highest temperatures led to hypomethylation of both embryonal masses and somatic plants. Moreover, we detected for the first time in a pine species the presence of 5-hydroxymethylcytosine, and revealed its tissue specificity and potential involvement in heat-stress responses. Additionally, a heat shock protein-coding gene showed a down-regulation tendency along the process, with a special emphasis given to embryonal masses at first subculture and ex vitro somatic plants. Likewise, the transcripts of several proteins related with translation, oxidative stress response, and drought resilience were differentially expressed. | en |
| dc.description.sponsorship | This research was funded by MINECO project (AGL2016-76143-C4-3R), CYTED (P117RT0522), DECO (Basque government) and MULTIFOREVER project, supported under the umbrella of ERA-NET Cofund ForestValue by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI) and VINNOVA (SE). ForestValue has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N◦773324. | en |
| dc.format.extent | 16 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.3390/plants9121762 | |
| dc.identifier.issn | 2223-7747 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/39931 | |
| dc.language.iso | eng | en |
| dc.publisher | MDPI | en |
| dc.relation.ispartof | Plants, 2020, 9, 1762 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/773324/ | |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76143-C4-3R/ | |
| dc.relation.publisherversion | https://doi.org/10.3390/plants9121762 | |
| dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | 5-Hydroxymethylcytosine | en |
| dc.subject | 5-Methylcytosine | en |
| dc.subject | Epigenetics | en |
| dc.subject | Heat | en |
| dc.subject | Heat shock protein | en |
| dc.subject | Memory | en |
| dc.subject | Pinus radiata | en |
| dc.subject | Priming | en |
| dc.subject | Somatic embryo | en |
| dc.subject | Somatic plant | en |
| dc.title | Induction of radiata pine somatic embryogenesis at high temperatures provokes a long-term decrease in DNA methylation/hydroxymethylation and differential expression of stress-related genes | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d | |
| relation.isAuthorOfPublication | e87ff19e-9d36-4286-989b-cafd391dff9d | |
| relation.isAuthorOfPublication.latestForDiscovery | 77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d |