Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures
| dc.contributor.author | Castander Olarieta, Ander | |
| dc.contributor.author | Moncaleán, Paloma | |
| dc.contributor.author | Pereira, Catia | |
| dc.contributor.author | Pěnčík, A. | |
| dc.contributor.author | Petřík, I. | |
| dc.contributor.author | Pavlović, I. | |
| dc.contributor.author | Novák, Ondrej | |
| dc.contributor.author | Strnad,Miroslav | |
| dc.contributor.author | Goicoa Mangado, Tomás | |
| dc.contributor.author | Ugarte Martínez, María Dolores | |
| dc.contributor.author | Montalbán, Itziar A. | |
| dc.contributor.department | Estadística, Informática y Matemáticas | es_ES |
| dc.contributor.department | Estatistika, Informatika eta Matematika | eu |
| dc.date.accessioned | 2022-02-01T11:27:18Z | |
| dc.date.available | 2022-02-01T11:27:18Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Vegetative propagation through somatic embryogenesis is an effective method to produce elite varieties and can be applied as a tool to study the response of plants to different stresses. Several studies show that environmental changes during embryogenesis could determine future plant development. Moreover, we previously reported that physical and chemical conditions during somatic embryogenesis can determine the protein, hormone and metabolite profiles, as well as the micromorphological and ultrastructural organization of embryonal masses and somatic embryos. In this sense, phytohormones are key players throughout the somatic embryogenesis process as well as during numerous stress-adaptation responses. In this work, we first applied different higherature regimes (30 °C, 4 weeks; 40 °C, 4 days; 50 °C, 5 min) during induction of Pinus radiata D. Don somatic embryogenesis, together with control temperature (23 °C). Then, the somatic plants regenerated from initiated embryogenic cell lines and cultivated in greenhouse conditions were subjected to drought stress and control treatments to evaluate survival, growth and several physiological traits (relative water content, water potential, photosynthesis, stomatal conductance and transpiration). Based on those preliminary results, even more extreme higherature regimes were applied during induction (40 °C, 4 h; 50 °C, 30 min; 60 °C, 5 min) and the corresponding cytokinin profiles of initiated embryonal masses from different lines were analysed. The results showed that the temperature regime during induction had delayed negative effects on drought resilience of somatic plants as indicated by survival, photosynthetic activity and water- use efficiency. However, high temperatures for extended periods of time enhanced subsequent plant growth in well-watered conditions. Higherature regime treatments induced significant differences in the profile of total cytokinin bases, N6-isopentenyladenine, cis-zeatin riboside and trans-zeatin riboside. We concluded that phytohormones could be potential regulators of stress-response processes during initial steps of somatic embryogenesis and that they may have delayed implications in further developmental processes, determining the performance of the generated plants. | en |
| dc.description.sponsorship | This research was funded by MINECO (Spanish Government) project (AGL2016-60476143-C4-3R), CYTED (P117RT0522), DECO (Basque government, ‘Ayudas de formación a jóvenes investigadores y tecnólogos’) and MULTIFOREVER (Forest Value, ERANET program, EU). Project MULTIFOREVER is supported under the umbrella of ERA-NET Cofund ForestValue by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI) and VINNOVA (SE). ForestValue has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°773324. This work was also supported by the Internal Grant Agency of Palacký University (IGA_PrF_2019_020) and from European Regional Development Fund-Project ‘Plants as a tool for sustainable global development’ (No. CZ.02.1.01/0.0/0.0/16_019/0000827). | en |
| dc.format.extent | 48 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Ander Castander-Olarieta, Paloma Moncaleán, Catia Pereira, Aleš Pěnčík, Ivan Petřík, Iva Pavlović, Ondřej Novák, Miroslav Strnad, Tomas Goicoa, Maria D Ugarte, Itziar A Montalbán, Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures, Tree Physiology, Volume 41, Issue 6, June 2021, Pages 912–926, https://doi.org/10.1093/treephys/tpaa055 | en |
| dc.identifier.doi | 10.1093/treephys/tpaa055 | |
| dc.identifier.issn | 0829-318X | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/42090 | |
| dc.language.iso | eng | en |
| dc.publisher | Oxford University Press | en |
| dc.relation.ispartof | Tree Physiology, Vol. 41, Issue 6, June 2021, pp. 912–926 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-60476143-C4-3R/ | |
| dc.relation.projectID | info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/773324/ | |
| dc.relation.publisherversion | https://doi.org/10.1093/treephys/tpaa055 | |
| dc.rights | © 2020 The Author(s) 2020. All rights reserved | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Delayed effects | en |
| dc.subject | Photosynthesis | en |
| dc.subject | Phytohormones | en |
| dc.subject | Radiata pine | en |
| dc.subject | Somatic plants | en |
| dc.subject | Stress | en |
| dc.subject | Water potential | en |
| dc.title | Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d | |
| relation.isAuthorOfPublication | e87ff19e-9d36-4286-989b-cafd391dff9d | |
| relation.isAuthorOfPublication.latestForDiscovery | 77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d |