AtCube: performing pathogen-root infection tests on Arabidopsis thaliana in a completely controlled way

Poveda Arias, Jorge

Publication:
AtCube: performing pathogen-root infection tests on Arabidopsis thaliana in a completely controlled way

dc.contributor.author	Poveda Arias, Jorge
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2022-04-07T07:47:48Z
dc.date.available	2022-04-07T07:47:48Z
dc.date.issued	2022
dc.description.abstract	Arabidopsis thaliana represents the main model plant for the experimental analysis of plant genetics and developmental biology of photoautotrophic organisms, due to characteristics such as size, life cycle, fecundity, genetics and its easy experimental manipulation. In turn, A. thaliana is used as a model plant in plant-microorganism interaction studies, being of great importance in the knowledge of the form of infection of numerous plant pathogens. The present work is based on the development of an A. thaliana plant growth system in Phytatray II boxes and culture substrate, trying to establish a system that is free of contamination, completely controlled, self-sufficient, reproducible and standardized, called AtCube. Through the use of different necrotrophic and biotrophic/hemibiotrophic root-pathogens, bacteria, fungi and oomycetes, and the comparison with a conventional system of growth in plots, various results are analyzed on the effects of pathogens on plants and the advantages and differences of the AtCube system. This work makes it possible to highlight that the AtCube system represents a methodology that allows obtaining results similar to other systems but with important advantages regarding its standardization, rigor and reproducibility with respect to studies with A. thaliana and root pathogens. Furthermore, it could represent an equally efficient system in studies with other plant species and the application of foliar pathogens and/or beneficial microorganisms.	en
dc.description.sponsorship	This work was supported by the Instituto de Estudios Zamoranos 'Florián de Ocampo' (IEZFO), in the XXVII and XXVIII Call for Research Grants, corresponding to the years 2019 and 2020.	en
dc.format.extent	8 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1016/j.pmpp.2021.101780
dc.identifier.issn	0885-5765
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/42682
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Physiological and Molecular Plant Pathology, 117 (2022) 101780	en
dc.relation.publisherversion	https://doi.org/10.1016/j.pmpp.2021.101780
dc.rights	© 2021 The Author. This is an open access article under the CC BY license.	es_ES
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Arabidopsis thaliana	en
dc.subject	Defense-related genes	en
dc.subject	Growth chamber	en
dc.subject	Reactive oxygen species	en
dc.subject	Root-pathogen	en
dc.title	AtCube: performing pathogen-root infection tests on Arabidopsis thaliana in a completely controlled way	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	85d07390-8e9d-444e-a969-87fff3f1497e