Post-harvest light treatment increases expression levels of recombinant proteins in transformed plastids of potato tubers
| dc.contributor.author | Larraya Reta, Luis María | |
| dc.contributor.author | Fernández San Millán, Alicia | |
| dc.contributor.author | Ancín Rípodas, María | |
| dc.contributor.author | Farrán Blanch, Inmaculada | |
| dc.contributor.author | Veramendi Charola, Jon | |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.date.accessioned | 2018-04-10T13:08:14Z | |
| dc.date.available | 2018-04-10T13:08:14Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Plastid genetic engineering represents an attractive system for the production of foreign proteins in plants. Although high expression levels can be achieved in leaf chloroplasts, the results for non-photosynthetic plastids are generally discouraging, mainly due to low transcriptional and translational rates in comparison with chloroplasts. Here, we report the expression of two thioredoxin genes (trx f and m) from the potato plastid genome to study transgene expression in amyloplasts. As expected, the highest transgene expression was detected in the leaf (up to 4.2% of TSP). The Trx protein content in the tuber was approximately 2-3 orders of magnitude lower than in the leaf. However, we demonstrate that a simple post-harvest light treatment of microtubers developed in vitro or soil-grown tubers induces up to 55 times higher accumulation of the recombinant protein in just 7-10 days. The promoter and 5’-UTR of the psbA gene displayed higher light induction than the rrn promoter. After the applied treatment, the Trx f levels in microtubers and soil-grown tubers increased to 0.14% and 0.11% of TSP, respectively. Moreover, tubers stored for 8 months maintained the capacity of increasing the foreign protein levels after the light treatment. Post-harvest cold induction (up to 5 times) at 4 ºC was also detected in microtubers. We conclude that plastid transformation and post-harvest light treatment could be an interesting approach for the production of foreign proteins in potato. | en |
| dc.description.sponsorship | This work was supported by Grant AGL2010-15107 from the Ministerio de Ciencia e Innovación (Spain). M.A. is a recipient of a FPU fellowship from the Ministerio de Educación, Cultura y Deporte (Spain). | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1002/biot.201500028 | |
| dc.identifier.issn | 1860-6768 (Print) | |
| dc.identifier.issn | 1860-7314 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/28229 | |
| dc.language.iso | eng | en |
| dc.publisher | Wiley | en |
| dc.relation.ispartof | Biotechnology Journal, Vol. 10, Issue 11, November 2015, pgs. 1803-1813 | en |
| dc.relation.publisherversion | https://doi.org/10.1002/biot.201500028 | |
| dc.rights | © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Amyloplast | en |
| dc.subject | Microtuber | en |
| dc.subject | Plastid transformation | en |
| dc.subject | Potato | en |
| dc.subject | Thioredoxin | en |
| dc.title | Post-harvest light treatment increases expression levels of recombinant proteins in transformed plastids of potato tubers | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | |
| dspace.entity.type | Publication | |
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