Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance

Osés Recalde, Maddi; Medrano Echeverría, María; Margareto, Javier; Portillo, María P.; Aguilera, Concepción María; Altmäe, Signe; Labayen Goñi, Idoia

Publication:
Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance

dc.contributor.author	Osés Recalde, Maddi
dc.contributor.author	Medrano Echeverría, María
dc.contributor.author	Margareto, Javier
dc.contributor.author	Portillo, María P.
dc.contributor.author	Aguilera, Concepción María
dc.contributor.author	Altmäe, Signe
dc.contributor.author	Labayen Goñi, Idoia
dc.contributor.department	Institute on Innovation and Sustainable Development in Food Chain - ISFOOD	en
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.date.accessioned	2023-01-19T11:02:32Z
dc.date.available	2023-01-19T11:02:32Z
dc.date.issued	2022
dc.date.updated	2023-01-19T10:29:20Z
dc.description.abstract	Background: miRNA have been proposed as potential biomarkers of metabolic diseases. Objectives: To identify potential miRNA biomarkers of early metabolic-associated fatty liver disease (MAFLD) and/or insulin resistance (IR) in preadolescent children. Methods: A total of 70 preadolescents, aged 8.5–12 years old participated in the study. Hepatic fat was assessed by magnetic resonance imaging. Fasting blood biochemical parameters were measured and HOMA-IR calculated. Peripheral blood mononuclear cells (PBMC)-derived miRNA profiles associated with MAFLD (≥5.5% hepatic fat) and IR (HOMA-IR ≥2.5) were identified using untargeted high-throughput miRNAs sequencing (RNA-seq). Results: A total of 2123 PBMC-derived miRNAs were identified in children with (21.4%) or without MAFLD. Among them, hsa-miR-143-3p, hsa-miR-142-5p and hsamiR-660-5p were up-regulated, and p-hsa-miR-247, hsa-let-7a-5p and hsa-miR6823-3p down-regulated. Importantly, children with MAFLD had consistently higher miR-660-5p expression levels than their peers without it (p < 0.01), regardless of weight status. A total of 2124 PBMC-derived miRNA were identified in children with IR (28.6%) versus children without IR, where thirteen of them were dysregulated (p < 0.05) in children with IR. In addition, children with IR showed higher levels of miR-374a-5p and miR-190a-5p (p < 0.01) and lower levels of miR-4284 and miR4791 (p < 005), than their peers without IR in both the whole sample and in those with overweight or obesity. Conclusions: Our study results suggest circulating miR-660-5p as a potential biomarker of the presence of MAFLD in preadolescent children while circulating miR320a, miR-142-3p, miR-190a-5p, miR-374a-5p and let-7 family miRNAs could serve as potential biomarkers of IR in children.	en
dc.description.sponsorship	This project was funded by the Spanish Ministry of Health “Fondos de Investigación Sanitaria del Instituto de Salud Carlos III” (PI13/01335), the Spanish Ministry of Industry and Competitiveness (DEP2016-78377-R), by EU Fondos Estructurales de la Unión Europea (FEDER) funds (“Una manera de hacer Europa”) and Department of Economic Development of Government of Navarra (0011–1365–2019-000000, 0011–1365–2020-00140). Signe Altmae is supported by Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and European Regional Development Fund (FEDER) grant RYC 2016 21199. Maddi Oses is supported by a grant from the Spanish Ministry of Economy and Competitiveness, grant number; BES-2017-080770.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Oses, M., Medrano, M., Margareto Sanchez, J., Portillo, M. P., Aguilera, C. M., Altmäe, S., & Labayen, I. (2022). Peripheral blood mononuclear cells‐expressed miRNA profiles derived from children with metabolic‐associated fatty liver disease and insulin resistance. Pediatric Obesity, 17(12). https://doi.org/10.1111/ijpo.12966	en
dc.identifier.doi	10.1111/ijpo.12966
dc.identifier.issn	2047-6302
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44591
dc.language.iso	eng	en
dc.publisher	Wiley	en
dc.relation.ispartof	Pediatric Obesity 2022;17:e12966	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//PI13%2F01335/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI//DEP2016-78377-R	en
dc.relation.projectID	info:eu-repo/grantAgreement///RYC-2016-21199	en
dc.relation.projectID	info:eu-repo/grantAgreement///BES-2017-080770	en
dc.relation.publisherversion	https://doi.org/10.1111/ijpo.12966
dc.rights	© 2022 The Authors. This is an open access article under the terms of theCreative Commons Attribution-NonCommercialLicense, which permits use, distribution and reproduction in anymedium, provided the original work is properly cited and is not used for commercial purposes.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/
dc.subject	Children	en
dc.subject	Fatty liver	en
dc.subject	Insulin resistance	en
dc.subject	Metabolic diseases	en
dc.subject	MiRNA	en
dc.subject	Obesity	en
dc.title	Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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