Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda

Ramírez-Arias, Fernando G.; Lasa, Rodrigo; Murillo Pérez, Rosa; Navarro de la Fuente, Laura; Mercado, Gabriel; Williams, Trevor

Publication:
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda

dc.contributor.author	Ramírez-Arias, Fernando G.
dc.contributor.author	Lasa, Rodrigo
dc.contributor.author	Murillo Pérez, Rosa
dc.contributor.author	Navarro de la Fuente, Laura
dc.contributor.author	Mercado, Gabriel
dc.contributor.author	Williams, Trevor
dc.contributor.department	Agronomía, Biotecnología y Alimentación	es_ES
dc.contributor.department	Agronomia, Bioteknologia eta Elikadura	eu
dc.date.accessioned	2020-01-07T12:20:44Z
dc.date.available	2020-08-01T23:00:17Z
dc.date.issued	2019
dc.description.abstract	The inefficient production of nucleopolyhedrovirus occlusion bodies (OBs) can limit the commercialization of virus-based insecticides. The production of OBs in SfMNPV-infected Spodoptera frugiperda fourth instars was compared among groups of larvae that were frozen (−20 °C) immediately following death, or subjected to a 9-day period of post-mortem incubation at 5 °C or 15 °C. Incubation at 15 °C resulted in a ∼40% increase in total OB production and OBs per mg larval weight, compared to the −20 °C and 5 °C treatments. OBs from the 5 °C treatment were ∼10% smaller in cross sectional area and small OBs (<1 µm 2 ) were more abundant than in other treatments, possibly due to reduced post-mortem OB maturation in this treatment. SfMNPV genomic DNA in OB samples was 3.6-fold higher in the −20 °C treatment than the 5 °C treatment and 1.7-fold higher than the 15 °C treatment, possibly due to differences in the exposure of viral genomes to degradative enzymes. However, these differences did not affect the concentration-mortality relationship or speed of kill of OBs from the different treatments. The abundance of aerobic microbes increased from ∼3 × 10 7 CFU/ml in the −20 °C treatment, to approximately 2 × 10 8 and 3 × 10 8 CFU/ml in the 5 °C and 15 °C incubation treatments, respectively, similar to levels seen in other nucleopolyhedroviruses produced in insects. We conclude that post-mortem incubation at 15 °C likely involves continuing processes of virion occlusion and OB maturation that increase overall OB production without loss of insecticidal activity, although the value of this step in commercial virus insecticide production will depend on the cost of the incubation step and the value of the additional OBs produced.	en
dc.embargo.lift	2020-08-01
dc.embargo.terms	2020-08-01
dc.format.extent	36 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.biocontrol.2019.05.003
dc.identifier.issn	1049-9644
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/36007
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Biological Control 135 (2019) 33-40	en
dc.relation.publisherversion	https://doi.org/10.1016/j.biocontrol.2019.05.003
dc.rights	© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Spodoptera frugiperda multiple nucleopolyhedrovirus	en
dc.subject	Biological insecticide production	en
dc.subject	Insecticidal activity	en
dc.subject	qPCR	en
dc.subject	OB size	en
dc.title	Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	46f59760-02e7-4f4d-bfb2-e505b2875f48