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Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes

dc.contributor.authorEugui Arrizabalaga, Daniel
dc.contributor.authorVelasco, Pablo
dc.contributor.authorAbril Urías, Patricia
dc.contributor.authorEscobar, Carolina
dc.contributor.authorGómez-Torres, Óscar
dc.contributor.authorCaballero, Sara
dc.contributor.authorPoveda Arias, Jorge
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.date.accessioned2023-06-21T13:36:44Z
dc.date.available2023-06-21T13:36:44Z
dc.date.issued2023
dc.date.updated2023-06-21T13:27:42Z
dc.description.abstractThis study aimed to standarize a protocol for obtaining a bioactive extract from broccoli (Brassica oleracea var. italica) crop residues, that is suitable for application on an industrial scale and effective in reducing plant disease incidence. For this purpose, the influence of several extraction factors in the glucosinolate (GSL) content was studied with leaves collected from two conventional broccoli fields and two organic broccoli fields. The analysis showed that lyophilization had no influence on the GSL content. Storage of plant material under two different temperatures (− 20ºC and − 80ºC) had no influence on the GSLs content of the extracts. Phytotoxicity of the extracts was studied with six different plant seeds, and also cytotoxicity was determined with human liver cells in vitro. The extracts were phytotoxic at dilutions above 10%, while cell toxicity was low. Extracts concentrations of 0.1%, 1% and 2% were tested in vitro against eight plant pathogenic fungi and two oomycetes in solid and in liquid media. The extracts reduced the growth of several plant pathogenic fungi at 2% dilution by up to 38.37% against Alternaria alternata and up to 46.55% against Sclerotinia sclerotiorum. When combined with myrosinase enzyme the effect of the extracts was enhanced, reaching inhibition values of 67.06% against A. alternata in solid medium and 68.52% against Rhizoctonia solani in liquid medium. In contrast, the same extracts increased the growth of the plant pathogenic oomycetes Pythium ultimum and Phytophthora cactorum. The effect of the same extracts in the free leaving larvae, J2s, of the plant-parasitic nematode (PPN) Meloidogyne javanica was not obvious. Minor significant differences were obtained but with no clear dose-response in nematode mortality, and no inhibition of eggs hatching was observed. These results show the industrial potential of using broccoli residues to obtain extracts with biopesticide activity against plant pathogenic fungi.en
dc.description.sponsorshipD.E. is beneficiary of an Industrial Doctorate (DIN2018-009852) by the State Research Agency of Spain. Open Access funding provided by University of Valladolid.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.citationEugui, D., Velasco, P., Abril-Urías, P., Escobar, C., Gómez-Torres, Ó., Caballero, S., Poveda, J. (2023) Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes. Industrial Crops and Products, 200(Part A), 1-16. https://doi.org/10.1016/j.indcrop.2023.116841.en
dc.identifier.doi10.1016/j.indcrop.2023.116841
dc.identifier.issn0926-6690es_ES
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/45600
dc.language.isoengen
dc.publisherElsevieren
dc.relation.ispartofIndustrial Crops and Products 200 Part A (2023) 116841en
dc.relation.publisherversionhttps://doi.org/10.1016/j.indcrop.2023.116841
dc.rights© 2023 The Authors. This is an open access article under the CC BY-NC-ND license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBrassicaceaeen
dc.subjectIsothiocyanatesen
dc.subjectMyrosinaseen
dc.subjectAlternariaen
dc.subjectSustainable agricultureen
dc.titleGlucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodesen
dc.typeinfo:eu-repo/semantics/article
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dspace.entity.typePublication
relation.isAuthorOfPublication5cae3dac-5b4b-47b1-93d0-0bf5611ed0c6
relation.isAuthorOfPublication85d07390-8e9d-444e-a969-87fff3f1497e
relation.isAuthorOfPublication.latestForDiscovery5cae3dac-5b4b-47b1-93d0-0bf5611ed0c6

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