Plasmid replicons from Pseudomonas are natural chimeras of functional, exchangeable modules

Bardají Goikoetxea, Leire; Añorga García, Maite; Ruiz Masó, José A.; Solar, Gloria del; Murillo Martínez, Jesús

Plasmid replicons from Pseudomonas are natural chimeras of functional, exchangeable modules

dc.contributor.author	Bardají Goikoetxea, Leire
dc.contributor.author	Añorga García, Maite
dc.contributor.author	Ruiz Masó, José A.
dc.contributor.author	Solar, Gloria del
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.date.accessioned	2017-02-14T13:15:31Z
dc.date.available	2017-02-14T13:15:31Z
dc.date.issued	2017
dc.description	Incluye ficheros de datos	es_ES
dc.description.abstract	Plasmids are a main factor for the evolution of bacteria through horizontal gene exchange, including the dissemination of pathogenicity genes, resistance to antibiotics and degradation of pollutants. Their capacity to duplicate is dependent on their replication determinants (replicon), which also define their bacterial host range and the inability to coexist with related replicons. We characterize a second replicon from the virulence plasmid pPsv48C, from Pseudomonas syringae pv. savastanoi, which appears to be a natural chimera between the gene encoding a newly described replication protein and a putative replication control region present in the widespread family of PFP virulence plasmids. We present extensive evidence of this type of chimerism in structurally similar replicons from species of Pseudomonas, including environmental bacteria as well as plant, animal and human pathogens. We establish that these replicons consist of two functional modules corresponding to putative control (REx-C module) and replication (REx-R module) regions. These modules are functionally separable, do not show specificity for each other, and are dynamically exchanged among replicons of four distinct plasmid families. Only the REx-C module displays strong incompatibility, which is overcome by a few nucleotide changes clustered in a stem-and-loop structure of a putative antisense RNA. Additionally, a REx-C module from pPsv48C conferred replication ability to a non-replicative chromosomal DNA region containing features associated to replicons. Thus, the organization of plasmid replicons as independent and exchangeable functional modules is likely facilitating rapid replicon evolution, fostering their diversification and survival, besides allowing the potential co-option of appropriate genes into novel replicons and the artificial construction of new replicon specificities.	en
dc.description.sponsorship	This work was funded by the Spanish Plan Nacional I+D+i grant AGL2014-53242-C2-2-R, from the Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER). M.A. was supported by an FPI fellowship (reference BES-2012-054016, Ministerio de Ciencia e Innovación/Ministerio de Economía y Competitividad, Spain).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3389/fmicb.2017.00190
dc.identifier.issn	1664-302X (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/23654
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Microbiology 8: 190	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2014-53242-C2-2-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30343-C02-02/ES/
dc.relation.publisherversion	https://doi.org/10.3389/fmicb.2017.00190
dc.rights	© 2017 Bardaji, Añorga, Ruiz-Masó, del Solar and Murillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Control and replication modules	en
dc.subject	Chimeric replicons	en
dc.subject	Gene co-option	en
dc.subject	Rep proteins	en
dc.subject	Origin of replication	en
dc.subject	Plasmid incompatibility	en
dc.subject	Swapping of functional modules	en
dc.subject	Virulence plasmids	en
dc.title	Plasmid replicons from Pseudomonas are natural chimeras of functional, exchangeable modules	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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