Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population

Barrera Cubillos, Gloria Patricia; Williams, Trevor; Villamizar, Laura; Caballero Murillo, Primitivo; Simón de Goñi, Oihane

Publication:
Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population

dc.contributor.author	Barrera Cubillos, Gloria Patricia
dc.contributor.author	Williams, Trevor
dc.contributor.author	Villamizar, Laura
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.author	Simón de Goñi, Oihane
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-09-06T12:20:18Z
dc.date.available	2018-09-06T12:20:18Z
dc.date.issued	2013
dc.description.abstract	A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 plaque purified virus were characterized. Ten distinct genotypes were identified (named A through J). SfCOL-A was the most prevalent (71±2%; mean ± SE) showing a PstI restriction profile indistinguishable to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8 - 21.8 Kb located mainly between nucleotides 11,436 and 33,883 in the reference genome SfMNPV-B, affecting the region between open reading frames (ORFs) sf20 and sf33. The insecticidal activity of each genotype from SfCOL-wt and several mixtures of genotypes was compared to that of SfCOL-wt. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of genotype mixtures cooccluded within OBs were consistently reduced in two-genotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or -F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes, or mixtures thereof. Deleted genotypes reduced OB potency but increased OB production of the SfCOL-wt population, which is structured to maximize the production of OBs in each infected host.	en
dc.description.sponsorship	This study received financial support from CSIC–COLCIENCIAS Cooperation project 2008CO0029 and the Colombian Ministry of Agriculture and Rural Development (Project 007X4648-176-968).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0077271
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30545
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 8(10): e77271	en
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0077271
dc.rights	© 2013 Barrera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)	en
dc.subject	Insecticidial properties	en
dc.subject	Deletion genotypes	en
dc.title	Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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