Remarkably efficient production of a highly insecticidal Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) isolate in its homologous host
| dc.contributor.author | Bernal Rodríguez, Alexandra | |
| dc.contributor.author | Simón de Goñi, Oihane | |
| dc.contributor.author | Williams, Trevor | |
| dc.contributor.author | Muñoz Labiano, Delia | |
| dc.contributor.author | Caballero Murillo, Primitivo | |
| dc.contributor.department | Producción Agraria | es_ES |
| dc.contributor.department | Nekazaritza Ekoizpena | eu |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.date.accessioned | 2025-01-31T10:14:21Z | |
| dc.date.available | 2025-01-31T10:14:21Z | |
| dc.date.issued | 2018-01-03 | |
| dc.date.updated | 2025-01-31T10:02:03Z | |
| dc.description | Acceso cerrado a este documento. No se encuentra disponible para la consulta pública. Depositado en Academica-e para cumplir con los requisitos de evaluación y acreditación académica del autor/a (sexenios, acreditaciones, etc.). | es_ES |
| dc.description.abstract | Background: a Chrysodeixis chalcites nucleopolyhedrovirus from the Canary Islands (ChchNPV-TF1) has proved to be effective for control of Chrysodeixis chalcites on banana crops. Commercialization of this virus as a bioinsecticide requires an efficient production system. Results: the sixth instar (L6) was the most suitable for virus production, producing 1.80 × 1011 occlusion bodies (OB)/larva and showed a lower prevalence of cannibalism (5.4%) than fourth (L4) or fifth (L5) instars. Inoculation of L6 at 24 h post molting produced six times more OB (5.72 × 1011 OB/larva) than recently molted L6 larvae (1.00 × 1011 OB/larva). No significant differences were recorded in mean time to death (165–175 h) or OB production per larva (3.75 × 1011 to 5.97 × 1011) or per mg larval weight (1.30 × 1011 to 2.11 × 109), in larvae inoculated with a range of inoculum concentrations (LC50–LC90). Groups of infected L6 larvae reared at a density of 150 larvae/container produced a greater total number of OBs (8.07 × 1013 OB/container) than lower densities (25, 50 and 100 OB/container), and a similar number to containers with 200 inoculated larvae (8.43 × 1013 OB/container). Conclusion: the processes described here allow efficient production of sufficient OBs to treat ∼ 40 ha of banana crops using the insects from a single container. | en |
| dc.description.sponsorship | his study received financial support from the Instituto Nacional de Investigaciones Agrarias (Project RTA2010-00016-C02-02 and RTA2013-00114-C02-02) and the Programa Nacional Español de I + D + I (Projects AGL2008-05456-CO3-01 and AGL2011-30352-CO2-01). | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Bernal-Rodríguez, A., Simón-de Goñi, O., Williams, T., Muñoz-Labiano, D., Caballero-Murillo, P. (2018) Remarkably efficient production of a highly insecticidal Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) isolate in its homologous host. Pest Management Science, 74(7), 1586-1592. https://doi.org/10.1002/ps.4846 | |
| dc.identifier.doi | 10.1002/ps.4846 | |
| dc.identifier.issn | 1526-498X | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/53229 | |
| dc.language.iso | eng | |
| dc.publisher | Wiley | |
| dc.relation.ispartof | Pest Management Science, 74(7), 1586-1592 | |
| dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//RTA2010-00016-C02-02/ES/ | |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//RTA2013-00114-C02-02/ES/ | |
| dc.relation.projectID | info:eu-repo/grantAgreement/MICYT/CICYT/AGL2008-05456-CO3-01/ | |
| dc.relation.projectID | info:eu-repo/grantAgreement/MICYT/CICYT/AGL2011-30352-CO2-01/ | |
| dc.relation.publisherversion | https://doi.org/10.1002/ps.4846 | |
| dc.rights | © 2018 Society of Chemical Industry | |
| dc.rights.accessRights | info:eu-repo/semantics/closedAccess | |
| dc.subject | Chrysodeixis chalcites | en |
| dc.subject | Alphabaculovirus | en |
| dc.subject | ChchNPV | en |
| dc.subject | In vivo virus production | en |
| dc.subject | Biological insecticide | en |
| dc.subject | Cannibalism | en |
| dc.title | Remarkably efficient production of a highly insecticidal Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) isolate in its homologous host | en |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication | |
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