Somatic transposition and meiotically driven elimination of an active helitron family in Pleurotus ostreatus

Borgognone, Alessandra; Castanera Andrés, Raúl; Muguerza Domínguez, Elaia; Pisabarro de Lucas, Gerardo; Ramírez Nasto, Lucía

Somatic transposition and meiotically driven elimination of an active helitron family in Pleurotus ostreatus

dc.contributor.author	Borgognone, Alessandra
dc.contributor.author	Castanera Andrés, Raúl
dc.contributor.author	Muguerza Domínguez, Elaia
dc.contributor.author	Pisabarro de Lucas, Gerardo
dc.contributor.author	Ramírez Nasto, Lucía
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2018-09-04T06:43:43Z
dc.date.available	2018-09-04T06:43:43Z
dc.date.issued	2017
dc.description.abstract	Helitrons constitute a superfamily of DNA transposons that were discovered in silico and are widespread in most eukaryotic genomes. They are postulated to mobilize through a “rollingcircle” mechanism, but the experimental evidence of their transposition has been described only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families in meiotically derived progeny of the basidiomycete Pleurotus ostreatus. We found distorted segregation patterns of HELPO2 helitrons that led to a strong under-representation of these elements in the progeny. Further investigation of HELPO2 flanking sites showed that gene conversion may contribute to the elimination of such repetitive elements in meiosis, favouring the presence of HELPO2 vacant loci. In addition, the analysis of HELPO2 content in a reconstructed pedigree of subclones maintained under different culture conditions revealed an event of helitron somatic transposition. Additional analyses of genome and transcriptome data indicated that P. ostreatus carries active RNAi machinery that could be involved in the control of transposable element proliferation. Our results provide the first evidence of helitron mobilization in the fungal kingdom and highlight the interaction between genome defence mechanisms and invasive DNA.	en
dc.description.sponsorship	This work was supported by Spanish National Research Plan (Projects AGL2011-30495 and AGL2014-55971-R) and FEDER funds; Public University of Navarre (http://www.unavarra.es); AB holds a PhD scholarship from the Public University of Navarre and RC holds a FPI-PhD scholarship from the Ministry of Economy and Competitiveness.	en
dc.format.extent	13 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1093/dnares/dsw060
dc.identifier.issn	1340-2838,1756-1663
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30421
dc.language.iso	eng	en
dc.publisher	Oxford University Press	en
dc.relation.ispartof	DNA Research, 2017, 24(2), 103–115	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30495/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2014-55971-R/ES/
dc.relation.publisherversion	https://doi.org/10.1093/dnares/dsw060
dc.rights	© The Author(s) 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/
dc.subject	Helitrons	en
dc.subject	Segregation distortion	en
dc.subject	Gene conversion	en
dc.subject	Transposable elements	en
dc.subject	Basidiomycete	en
dc.title	Somatic transposition and meiotically driven elimination of an active helitron family in Pleurotus ostreatus	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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