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Whole exome sequencing characterization of individuals presenting extreme phenotypes of high and low risk of developing tobacco-induced lung adenocarcinoma

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dc.contributor.authorPatiño García, Ana
dc.contributor.authorGuruceaga, Elizabeth
dc.contributor.authorSegura, Víctor
dc.contributor.authorSánchez Bayona, Rodrigo
dc.contributor.authorAndueza, María Pilar
dc.contributor.authorTamayo Uria, Ibon
dc.contributor.authorSerrano, Guillermo
dc.contributor.authorFusco, Juan Pablo
dc.contributor.authorPajares Villandiego, María Josefa
dc.contributor.authorGúrpide, Alfonso
dc.contributor.authorOcón, Marimar
dc.contributor.authorSanmamed, Miguel F.
dc.contributor.authorRodríguez Ruiz, María
dc.contributor.authorMelero, Ignacio
dc.contributor.authorLozano, María Dolores
dc.contributor.authorAndrea, Carlos de
dc.contributor.authorPita, Guillermo
dc.contributor.authorGonzález Neira, Anna
dc.contributor.authorGonzález, Alvaro
dc.contributor.authorZulueta, Javier J.
dc.contributor.authorMontuenga, Luis M.
dc.contributor.authorPío, Rubén
dc.contributor.authorPérez Gracia, José Luis
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.date.accessioned2022-01-17T12:48:02Z
dc.date.available2022-01-17T12:48:02Z
dc.date.issued2021
dc.description.abstractBackground: tobacco is the main risk factor for developing lung cancer. Yet, some heavy smokers do not develop lung cancer at advanced ages while others develop it at young ages. Here, we assess for the first time the genetic background of these clinically relevant extreme phenotypes using whole exome sequencing (WES). Methods: we performed WES of germline DNA from heavy smokers who either developed lung adenocarcinoma at an early age ( extreme cases, n=50) or did not present lung adenocarcinoma or other tumors at an advanced age (extreme controls, n=50). We selected non-synonymous variants located in exonic regions and consensus splice sites of the genes that showed significantly different allelic frequencies between both cohorts. We validated our results in all the additional extreme cases (i.e., heavy smokers who developed lung adenocarcinoma at an early age) available from The Cancer Genome Atlas (TCGA). Results: the mean age for the extreme cases and controls was respectively 49.7 and 77.5 years. Mean tobacco consumption was 43.6 and 56.8 pack-years. We identified 619 significantly different variants between both cohorts, and we validated 108 of these in extreme cases selected from TCGA. Nine validated variants, located in relevant cancer related genes, such as PARP4, HLA-A or NQO1, among others, achieved statistical significance in the False Discovery Rate test. The most significant validated variant (P=4.48x10(-5)) was located in the tumor-suppressor gene ALPK2. Conclusions: we describe genetic variants associated with extreme phenotypes of high and low risk for the development of tobacco-induced lung adenocarcinoma. Our results and our strategy may help to identify high-risk subjects and to develop new therapeutic strategies.en
dc.description.sponsorshipThis work was supported by the Spanish Society of Medical Oncology; Fundación SEOM and Fundación Salud 2000; and Government of Navarra. LMM research work is supported by Foundation for Applied Medical Research (FIMA), Fundación Científica de la Asociación Espanola Contra el Cáncer, Fundación Ramón Areces, and Fondo de Investigación Sanitaria-Fondo Europeo de Desarrollo Regional 'Una manera de hacer Europa' (PI19/00098).en
dc.format.extent15 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.21037/tlcr-20-1197
dc.identifier.issn2226-4477
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/41809
dc.language.isoengen
dc.publisherAME Publishing
dc.relation.ispartofTranslational Lung Cancer Research, 10 (3), 1327-133
dc.relation.publisherversionhttp://doi.org/10.21037/tlcr-20-1197
dc.rights© Translational Lung Cancer Research. Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCancer risken
dc.subjectExtreme phenotypesen
dc.subjectWhole exome sequencing (WES)en
dc.subjectTobaccoen
dc.subjectLung adenocarcinomaen
dc.subjectALPK2en
dc.subjectHLA-Aen
dc.subjectPARP4en
dc.subjectNOQ1en
dc.titleWhole exome sequencing characterization of individuals presenting extreme phenotypes of high and low risk of developing tobacco-induced lung adenocarcinomaen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
relation.isAuthorOfPublication601df8fd-4b6f-47c4-be5d-4b81d2c1cf64
relation.isAuthorOfPublication.latestForDiscovery601df8fd-4b6f-47c4-be5d-4b81d2c1cf64

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