Alteration in the cerebrospinal fluid lipidome in Parkinson’s disease: a post-mortem pilot study

Fernández Irigoyen, Joaquín; Cartas Cejudo, Paz; Iruarrizaga-Lejarreta, Marta; Santamaría Martínez, Enrique

Alteration in the cerebrospinal fluid lipidome in Parkinson’s disease: a post-mortem pilot study

dc.contributor.author	Fernández Irigoyen, Joaquín
dc.contributor.author	Cartas Cejudo, Paz
dc.contributor.author	Iruarrizaga-Lejarreta, Marta
dc.contributor.author	Santamaría Martínez, Enrique
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.date.accessioned	2022-01-12T08:17:14Z
dc.date.available	2022-01-12T08:17:14Z
dc.date.issued	2021
dc.description.abstract	Lipid metabolism is clearly associated to Parkinson’s disease (PD). Although lipid homeostasis has been widely studied in multiple animal and cellular models, as well as in blood derived from PD individuals, the cerebrospinal fluid (CSF) lipidomic profile in PD remains largely unexplored. In this study, we characterized the post-mortem CSF lipidomic imbalance between neurologically intact controls (n = 10) and PD subjects (n = 20). The combination of dual extraction with ultra-performance liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC-ESI-qToF-MS/MS) allowed for the monitoring of 257 lipid species across all samples. Complementary multivariate and univariate data analysis identified that glycerolipids (mono-, di-, and triacylglycerides), saturated and mono/polyunsaturated fatty acids, primary fatty amides, glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines), sphingolipids (ceramides, sphingomyelins), N-acylethanolamines and sterol lipids (cholesteryl esters, steroids) were significantly increased in the CSF of PD compared to the control group. Interestingly, CSF lipid dyshomeostasis differed depending on neuropathological staging and disease duration. These results, despite the limitation of being obtained in a small population, suggest extensive CSF lipid remodeling in PD, shedding new light on the deployment of CSF lipidomics as a promising tool to identify potential lipid markers as well as discriminatory lipid species between PD and other atypical parkinsonisms.	en
dc.format.extent	20 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3390/biomedicines9050491
dc.identifier.issn	2227-9059
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/41737
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Biomedicines, 9 (5), 491	en
dc.relation.publisherversion	https://doi.org/10.3390/biomedicines9050491
dc.rights	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Lipids	en
dc.subject	Cerebrospinal fluid	en
dc.subject	Parkinson's disease	en
dc.subject	Mass-spectrometry	en
dc.subject	Lipidomics	en
dc.title	Alteration in the cerebrospinal fluid lipidome in Parkinson’s disease: a post-mortem pilot study	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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