Prokaryotic communities in the thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan salterns (Turkey) assessed by 16s rRNA amplicon sequencing

Akpolat, Can; Fernández González, Ana Beatriz; Caglayan, Pinar; Calli, Baris; Birbir, Meral; Ventosa, Antonio

Prokaryotic communities in the thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan salterns (Turkey) assessed by 16s rRNA amplicon sequencing

dc.contributor.author	Akpolat, Can
dc.contributor.author	Fernández González, Ana Beatriz
dc.contributor.author	Caglayan, Pinar
dc.contributor.author	Calli, Baris
dc.contributor.author	Birbir, Meral
dc.contributor.author	Ventosa, Antonio
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2022-01-18T09:18:41Z
dc.date.available	2022-01-18T09:18:41Z
dc.date.issued	2021
dc.description.abstract	Prokaryotic communities and physico-chemical characteristics of 30 brine samples from the thalassohaline Tuz Lake (Salt Lake), Deep Zone, Kayacik, Kaldirim, and Yavsan salterns (Turkey) were analyzed using 16S rRNA amplicon sequencing and standard methods, respectively. Archaea (98.41% of reads) was found to dominate in these habitats in contrast to the domain Bacteria (1.38% of reads). Representatives of the phylum Euryarchaeota were detected as the most predominant, while 59.48% and 1.32% of reads, respectively, were assigned to 18 archaeal genera, 19 bacterial genera, 10 archaeal genera, and one bacterial genus that were determined to be present, with more than 1% sequences in the samples. They were the archaeal genera Haloquadratum, Haloarcula, Halorhabdus, Natronomonas, Halosimplex, Halomicrobium, Halorubrum, Halonotius, Halolamina, Halobacterium, and Salinibacter within the domain Bacteria. The genera Haloquadratum and Halorhabdus were found in all sampling sites. While Haloquadratum, Haloarcula, and Halorhabdus were the most abundant genera, two uncultured Tuz Lake Halobacteria (TLHs) 1 and 2 were detected in high abundance, and an additional uncultured haloarchaeal TLH-3 was found as a minor abundant uncultured taxon. Their future isolation in pure culture would permit us to expand our knowledge on hyper-saline thalassohaline habitats, as well as their ecological role and biomedical and biotechnological potential applications.	en
dc.description.sponsorship	The authors would like to express their gratitude to Marmara University Scientific Research Projects Commission (BAPKO) for funding.	en
dc.format.extent	18 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3390/microorganisms9071525
dc.identifier.issn	2076-2607
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/41840
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Microorganisms 2021, 9, 1525	en
dc.relation.publisherversion	http://doi.org/10.3390/microorganisms9071525
dc.rights	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	16S rRNA amplicon sequencing	en
dc.subject	Extremophiles	en
dc.subject	Metagenomics	en
dc.subject	Physico-chemical analyses	en
dc.subject	Salterns	en
dc.subject	Thalassohaline lakes	en
dc.subject	Tuz Lake (Salt Lake)	en
dc.title	Prokaryotic communities in the thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan salterns (Turkey) assessed by 16s rRNA amplicon sequencing	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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