SarA positively controls Bap-dependent biofilm formation in Staphylococcus aureus

Trotonda, María Pilar; Manna, Adhar C.; Cheung, Ambrose L.; Lasa Uzcudun, Íñigo; Penadés, José R.

Publication:
SarA positively controls Bap-dependent biofilm formation in Staphylococcus aureus

dc.contributor.author	Trotonda, María Pilar
dc.contributor.author	Manna, Adhar C.
dc.contributor.author	Cheung, Ambrose L.
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.author	Penadés, José R.
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2019-01-21T13:24:44Z
dc.date.available	2019-01-21T13:24:44Z
dc.date.issued	2005
dc.description.abstract	The biofilm-associated protein Bap is a staphylococcal surface protein involved in biofilm formation. We investigated the influence of the global regulatory locus sarA on bap expression and Bap-dependent biofilm formation in three unrelated Staphylococcus aureus strains. The results showed that Bap-dependent biofilm formation was diminished in the sarA mutants by an agr-independent mechanism. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation. As expected, the diminished capacity to form biofilms in the sarA mutants correlated with the decreased presence of Bap in the bacterial surface. Using transcriptional fusion and Northern analysis data, we demonstrated that the sarA gene product acts as an activator of bap expression. Finally, the bap promoter was characterized and the transcriptional start point was mapped by the rapid amplification of cDNA ends technique. As expected, we showed that purified SarA protein binds specifically to the bap promoter, as determined by gel shift and DNase I footprinting assays. Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression (J. Valle, A. Toledo-Arana, C. Berasain, J. M. Ghigo, B. Amorena, J. R. Penades, and I. Lasa, Mol. Microbiol. 48:1075–1087), we propose that SarA is an essential regulator controlling biofilm formation in S. aureus.	en
dc.description.sponsorship	This work was supported by grant BIO2002-04542-C02-01 from the Comisión Interministerial de Ciencia y Tecnología (C.I.C.Y.T.) as well as grants from the Cardenal Herrera-CEU University, from the Conselleria de Agricultura, Pesca i Alimentació (CAPiA), and from the Generalitat Valenciana (CTIDIA/2002/62, CTESPP/2003/027 and AE04-8) to J.R.P., as well as grants from the NIH (AI37142 and AI47441) to A.L.C. Fellowship support for M. Pilar Trotonda from the Cardenal Herrera-CEU University is gratefully acknowledged.	en
dc.format.extent	9 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1128/jb.187.16.5790-5798.2005
dc.identifier.issn	0021-9193 (Print)
dc.identifier.issn	1098-5530 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/32029
dc.language.iso	eng	en
dc.publisher	American Society for Microbiology	en
dc.relation.ispartof	Journal of Bacteriology, Aug. 2005, Vol. 187, No. 16, p. 5790–5798	en
dc.relation.publisherversion	https://doi.org/10.1128/jb.187.16.5790-5798.2005
dc.rights	© 2005, American Society for Microbiology. All Rights Reserved.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.subject	Staphylococcus aureus	en
dc.subject	Bap-dependent biofilm formation	en
dc.subject	sarA	en
dc.title	SarA positively controls Bap-dependent biofilm formation in Staphylococcus aureus	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
relation.isAuthorOfPublication	c654d104-1ae2-41cf-9215-4b4bed3e5ea6
relation.isAuthorOfPublication.latestForDiscovery	c654d104-1ae2-41cf-9215-4b4bed3e5ea6