Noncontiguous operon is a genetic organization for coordinating bacterial gene expression

Sáenz Lahoya, S.; Bitarte Manzanal, Nerea; García, Beñat; Burgui Erice, Saioa; Vergara Irigaray, Marta; Valle Turrillas, Jaione; Solano Goñi, Cristina; Toledo Arana, Alejandro; Lasa Uzcudun, Íñigo

Publication:
Noncontiguous operon is a genetic organization for coordinating bacterial gene expression

dc.contributor.author	Sáenz Lahoya, S.
dc.contributor.author	Bitarte Manzanal, Nerea
dc.contributor.author	García, Beñat
dc.contributor.author	Burgui Erice, Saioa
dc.contributor.author	Vergara Irigaray, Marta
dc.contributor.author	Valle Turrillas, Jaione
dc.contributor.author	Solano Goñi, Cristina
dc.contributor.author	Toledo Arana, Alejandro
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2020-01-07T12:20:41Z
dc.date.available	2020-01-07T12:20:41Z
dc.date.issued	2019
dc.description.abstract	Bacterial genes are typically grouped into operons defined as clusters of adjacent genes encoding for proteins that fill related roles and are transcribed into a single polycistronic mRNA molecule. This simple organization provides an efficient mechanism to coordinate the expression of neighboring genes and is at the basis of gene regulation in bacteria. Here, we report the existence of a higher level of organization in operon structure that we named noncontiguous operon and consists in an operon containing a gene(s) that is transcribed in the opposite direction to the rest of the operon. This transcriptional architecture is exemplified by the genes menE-menC-MW1733-ytkD-MW1731 involved in menaquinone synthesis in the major human pathogen Staphylococcus aureus. We show that menE-menC-ytkD-MW1731 genes are transcribed as a single transcription unit, whereas the MW1733 gene, located between menC and ytkD, is transcribed in the opposite direction. This genomic organization generates overlapping transcripts whose expression is mutually regulated by transcriptional interference and RNase III processing at the overlapping region. In light of our results, the canonical view of operon structure should be revisited by including this operon arrangement in which cotranscription and overlapping transcription are combined to coordinate functionally related gene expression.	en
dc.description.sponsorship	This work was supported by the Spanish Ministry of Economy and Competitiveness Grants BIO2014-53530-R and BIO2017-83035-R (Agencia Española de Investigacion/Fondo Europeo de Desarrollo Regional, European Union). A.T.-A. is supported by the European Research Council under the European Union's Horizon 2020 research and innovation programme Grant Agreement 646869.	en
dc.format.extent	6 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1073/pnas.1812746116
dc.identifier.issn	0027-8424
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/36000
dc.language.iso	eng	en
dc.publisher	National Academy of Sciences	en
dc.relation.ispartof	PNAS January 29, 2019 116 (5) 1733-1738	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2014-53530-R/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83035-R/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/646869	en
dc.relation.publisherversion	https://doi.org/10.1073/pnas.1812746116
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.subject	Operon	en
dc.subject	Antisense transcription	en
dc.subject	Overlapping transcription	en
dc.subject	RNase III	en
dc.subject	Menaquinone	en
dc.title	Noncontiguous operon is a genetic organization for coordinating bacterial gene expression	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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