Effect of a topical formulation on infective viral load in lambs naturally infected with orf virus

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dc.contributor.authorLacasta, Delia
dc.contributor.authorReina Arias, Ramsés
dc.contributor.authorRuiz de Arcaute, Marta
dc.contributor.authorFerrer, Luis Miguel
dc.contributor.authorBenito, Alfredo Ángel
dc.contributor.authorTejedor, María Teresa
dc.contributor.authorEcheverría Garín, Irache
dc.contributor.authorRuiz, Héctor
dc.contributor.authorMartínez Cárdenas, Silvia
dc.contributor.authorWindsor, Peter Andrew
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.date.accessioned2024-08-27T14:12:48Z
dc.date.available2024-08-27T14:12:48Z
dc.date.issued2021
dc.date.updated2024-08-27T13:33:53Z
dc.description.abstractIntroduction: Orf is a highly contagious eruptive viral disease of the skin and mucosa of sheep and goats. Although vaccination with live or attenuated orf virus is the preferred option for disease control, the vaccine is unavailable in many countries. Treatment of orf lesions involves standard hygiene and in numerous cases, management of presumptive secondary infections with antibiotics, increasing risks of antimicrobial resistance (AMR). The wound dressing formulation Tri-Solfen® containing two local anaesthetics (lignocaine and bupivacaine), adrenaline and an antiseptic (cetrimide) in a gel formulation was developed for pain relief in sheep undergoing surgical husbandry procedures in Australia. Recently, TS therapy was found to reduce suffering and enhance recovery in cattle and buffalo with oral and skin lesions due to foot-and-mouth disease (FMD) virus infection. It was noted that TS has a low pH and is potentially viricidal, potentially aiding disease control. Methods: One-month-old lambs (n=14), naturally infected with orf, were recruited from a farm during a natural outbreak of the disease. The animals were selected at the early stages of the infection and randomly divided into two cohorts: Group A (n=11) treated with the topical wound gel formulation (TS); and Group B (n=3) an untreated control group. Swabs were obtained before treatment (T0) and on days one (T1), 3 (T2) and 5 (T3) post-treatment, then submitted to direct DNA extraction with real-time PCR quantification, plus incubation with primary tissue cultures from ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF). Results: Although no significant differences were found in the clinical progression of the lesions and PCR quantification (p=0.722) between these small cohorts, there was a significant difference (p<0.05) in reduction in infective viral load between the groups when assessed in OSF cell cultures between T0 and T3. Conclusion: These preliminary findings suggest that treatment of early stage lesions with this TS may reduce the infective viral load present in orf lesions.en
dc.description.sponsorshipThis research was supported by funding and product from the Australian company Animal Ethics Pty Ltd. The work was also supported by the Aragón Government and the European Social Fund (A15_17R, Construyendo Aragón 2016-20) and Project CONECTIM funded by Gobierno de Navarra (PC052-053).
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLacasta, D., Reina, R., Ruiz de Arcaute, M., Ferrer, L. M., Benito, A. A., Tejedor, M. T., Echeverria, I., Ruiz, H., Cardenas, S. M., Windsor, P. A. (2021) Effect of a topical formulation on infective viral load in lambs naturally infected with orf virus. Veterinary Medicine: Research and Reports, 12, 149-158. https://doi.org/10.2147/VMRR.S306355.
dc.identifier.doi10.2147/VMRR.S306355
dc.identifier.issn2230-2034
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/51452
dc.language.isoeng
dc.publisherTaylor and Francis Group
dc.relation.ispartofVeterinary Medicine: Research and Reports 2021, 12, 149-158
dc.relation.projectIDinfo:eu-repo/grantAgreement/Gobierno de Navarra//PC052-053/
dc.relation.publisherversionhttps://doi.org/10.2147/VMRR.S306355
dc.rights© 2021 Lacasta et al. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution. Non Commercial (unported, v3.0) License.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectSheepen
dc.subjectContagious ecthymaen
dc.subjectWound formulationen
dc.subjectLocal therapyen
dc.titleEffect of a topical formulation on infective viral load in lambs naturally infected with orf virus
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication5eb332ef-80cd-4c2b-942f-e48c9d3725f2
relation.isAuthorOfPublication2d290f27-c082-42fa-987a-7c90914e555b
relation.isAuthorOfPublication.latestForDiscovery2d290f27-c082-42fa-987a-7c90914e555b

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