The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder

Crespo Martínez, Sara; Sobczak, Miroslaw; Różańska, Elżbieta; Forneck, Astrid; Griesser, Michaela

The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder

dc.contributor.author	Crespo Martínez, Sara
dc.contributor.author	Sobczak, Miroslaw
dc.contributor.author	Różańska, Elżbieta
dc.contributor.author	Forneck, Astrid
dc.contributor.author	Griesser, Michaela
dc.contributor.department	Agronomía, Biotecnología y Alimentación	es_ES
dc.contributor.department	Agronomia, Bioteknologia eta Elikadura	eu
dc.date.accessioned	2022-08-19T09:37:35Z
dc.date.available	2022-08-19T09:37:35Z
dc.date.issued	2019
dc.date.updated	2022-08-19T09:14:04Z
dc.description.abstract	Berry Shrivel (BS) is a post-veraison physiological ripening disorder of grapevine berries. Its symptoms encompass low pH, reduced content of sugars and anthocyanins, and loss of turgor leading to berries shriveling. Evidence for the primary causes of BS is still speculative and anatomical studies are scarce. So far, anatomical studies have determined necrotic cells, degraded primary phloem cells and hardening of secondary phloem cells in the rachis of BS affected grapes. The picture is far from being complete. Herein we report in-depth analyses of the ultrastructure, anatomy and spatial elementary analysis of rachis and pedicel tissues of BS symptomatic grape clusters with different symptom severity. We hypothesize that structural changes in the vascular system of BS affected grape clusters could alter transport functions of the phloem tissue and contribute to the appearance of BS symptoms. By applying different microscopic techniques (LM, SEM, TEM and EDS) we found a number of anatomical differences in both, rachis and pedicels, between H and BS symptomatic grapes, which include: (i) extended areas of collapsed cells and cell wall thickenings in the secondary phloem in BS samples; (ii) reduced number of cell layers in the cambium in BS samples; (iii) higher rate of callose deposition on sieve plates that are additionally covered with a carbohydrate-like material in BS samples; and (iv) reduced (up to 60%) estimated sieve tube conductivity in BS samples.	en
dc.description.sponsorship	The project was supported by the bilateral exchange and cooperation program between Austria and Poland (WTZ) project no. PL 08/2015 (9196) and by the Austrian Science Fund (FWF) project no. P 28966-B29.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/msword	en
dc.identifier.citation	Crespo-Martínez, S.; Sobczak, M.; Ró¿a¿ska, E.; Forneck, A.; Griesser, M.. (2019). The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder. Micron. 116, 36-45.	en
dc.identifier.doi	10.1016/j.micron.2018.09.012
dc.identifier.issn	0968-4328
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/43825
dc.language.iso	eng	en
dc.relation.ispartof	Micron, 2019, 116, pp. 36-45	en
dc.relation.publisherversion	https://doi.org/10.1016/j.micron.2018.09.012
dc.rights	© 2018 The Authors. This is an open access article under the CC BY license	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Berry Shrivel	en
dc.subject	Phloem	en
dc.subject	Sieve element	en
dc.subject	Sieve tube conductivity	en
dc.title	The role of the secondary phloem during the development of the grapevine Berry Shrivel ripening disorder	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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