First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis)
| dc.contributor.author | Lana, Maite | |
| dc.contributor.author | Simón de Goñi, Oihane | |
| dc.contributor.author | Velasco, Pablo | |
| dc.contributor.author | Rodríguez, Víctor Manuel | |
| dc.contributor.author | Caballero Murillo, Primitivo | |
| dc.contributor.author | Poveda Arias, Jorge | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.date.accessioned | 2023-06-30T12:01:13Z | |
| dc.date.available | 2023-06-30T12:01:13Z | |
| dc.date.issued | 2023 | |
| dc.date.updated | 2023-06-30T11:49:46Z | |
| dc.description.abstract | Cotton leaf worm (Spodoptera littoralis) is a pest that produces important losses in horticultural and ornamental crops in greenhouse, being classified as quarantine pest A2 by EPPO. One of the strategies proposed to control agricultural pests in a health and environmentally friendly way is biological control with entomopathogenic fungi. The genus of filamentous fungi Trichoderma includes different species with direct (infection, antibiosis, anti-feeding, etc.) and indirect (systemic activation of plant defenses) insecticidal capacity, however, the species T. hamatum has never been described previously as entomopathogenic. In this work, the entomopathogenic capacity of T. hamatum on S. littoralis L3 larvae was analyzed by applying spores and fungal filtrates (topically and orally). Infection by spores was compared with the commercial entomopathogenic fungus Beauveria bassiana, obtaining similar results with respect to the production of larval mortality. Oral application of spores reported high mortality and fungal colonization of larvae, however, T. hamatum did not show chitinase activity when grown in the presence of S. littoralis tissues. Therefore, infection of S. littoralis larvae by T. hamatum is through natural openings such as mouth, anus or spiracles. With respect to the application of filtrates, only those obtained from the liquid culture of T. hamatum in contact with S. littoralis tissues reported a significant reduction in larval growth. Metabolomic analysis of the filtrates determined that the filtrate with insecticidal capacity presented the siderophore rhizoferrin in large quantities, which could be responsible for this activity. However, the production of this siderophore had never been previously described in Trichoderma and its insecticidal capacity was unknown. In conclusion, T. hamatum presents entomopathogenic capacity against S. littoralis larvae through the application of spores and filtrates, and both ways could be the basis for the development of efficient bioinsecticides against the pest. | en |
| dc.description.sponsorship | This research was funded by Gobierno de Navarra (PC093–094 FORINSEC) and Xunta de Galicia (IN607A 2021/03). Grants for the Recualification of the Spanish University System for 2021–2023, Public University of Navarra; Recualification Modality; Funded by the European Union – NextGenerationEU (J.P. beneficiary). Open Access funding provided by University of Valladolid. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Lana, M., Simón, O., Velasco, P., Rodríguez, V. M., Caballero, P., & Poveda, J. (2023). First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: Development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis). Microbiological Research, 270, 127334. https://doi.org/10.1016/j.micres.2023.127334 | en |
| dc.identifier.doi | 10.1016/j.micres.2023.127334 | |
| dc.identifier.issn | 0944-5013 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/45672 | |
| dc.language.iso | eng | en |
| dc.publisher | Elsevier | en |
| dc.relation.ispartof | Microbiological Research, 270 (2023) 127334 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/Gobierno de Navarra//PC093–094/ | |
| dc.relation.publisherversion | https://doi.org/10.1016/j.micres.2023.127334 | |
| dc.rights | © 2023 The Author(s). This is an open access article under the CC BY license. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Beauveria bassiana | en |
| dc.subject | Chitinase | en |
| dc.subject | Fungi entomopathogen | en |
| dc.subject | Rhizoferrin | en |
| dc.subject | Trichoderma | en |
| dc.title | First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis) | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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