Soybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1
| dc.contributor.author | Gopalasubramaniama, Sabarinathan K. | |
| dc.contributor.author | Kondapallib, Kalyan C. | |
| dc.contributor.author | Millán-Pacheco, César | |
| dc.contributor.author | Pastor, Nina | |
| dc.contributor.author | Stemmler, Timothy L. | |
| dc.contributor.author | Morán Juez, José Fernando | |
| dc.contributor.author | Arredondo-Peter, Raúl | |
| dc.contributor.department | Ciencias del Medio Natural | es_ES |
| dc.contributor.department | Natura Ingurunearen Zientziak | eu |
| dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua | es |
| dc.date.accessioned | 2019-06-07T11:00:07Z | |
| dc.date.available | 2019-06-07T11:00:07Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Ferrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+ is reduced to Hb2+ by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb13+, however the use of ITC and IEF techniques revealed that this interaction is weak. In silico modeling showed that predicted FLbR2 and native Hb13+ interact at the FAD-binding domain of FLbR2 and the CD-loop and helix F of Hb13+. | en |
| dc.description.sponsorship | This work was funded by CoNaCyT (project no. 42873Q), México (to R. A.-P.), Dept. of Education, Government of Navarre (no. 999–2006), Spain (to J. F. M.) and NIDDKD (DK068139), USA (to T. L. S.). | en |
| dc.format.extent | 13 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/33266 | |
| dc.language.iso | eng | en |
| dc.publisher | Simplex Academic Publishers | en |
| dc.relation.ispartof | ScienceJet 2013, 2: 33 | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Fluorescence quenching | en |
| dc.subject | Hemoglobin | en |
| dc.subject | Isothermal calorimetry | en |
| dc.subject | Non-symbiotic | en |
| dc.subject | Oryza sativa | en |
| dc.subject | Protein-protein interaction | en |
| dc.title | Soybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1 | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | cad13109-2660-4ec4-80c8-e4bcc0685382 | |
| relation.isAuthorOfPublication.latestForDiscovery | cad13109-2660-4ec4-80c8-e4bcc0685382 |