Functional analysis of intergenic regulatory regions of genes encoding surface adhesins in Staphylococcus aureus isolates from periprosthetic joint infections

Morales Laverde, Liliana Andrea; Trobos, Margarita; Echeverz Sarasúa, Maite; Solano Goñi, Cristina; Lasa Uzcudun, Íñigo

Functional analysis of intergenic regulatory regions of genes encoding surface adhesins in Staphylococcus aureus isolates from periprosthetic joint infections

dc.contributor.author	Morales Laverde, Liliana Andrea
dc.contributor.author	Trobos, Margarita
dc.contributor.author	Echeverz Sarasúa, Maite
dc.contributor.author	Solano Goñi, Cristina
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.date.accessioned	2023-02-24T07:42:16Z
dc.date.available	2023-02-24T07:42:16Z
dc.date.issued	2022
dc.date.updated	2023-02-24T07:29:27Z
dc.description.abstract	Staphylococcus aureus is a leading cause of prosthetic joint infections (PJI). Surface adhesins play an important role in the primary attachment to plasma proteins that coat the surface of prosthetic devices after implantation. Previous efforts to identify a genetic component of the bacterium that confers an enhanced capacity to cause PJI have focused on gene content, kmers, or single-nucleotide polymorphisms (SNPs) in coding sequences. Here, using a collection of S. aureus strains isolated from PJI and wounds, we investigated whether genetic variations in the regulatory region of genes encoding surface adhesins lead to differences in their expression levels and modulate the capacity of S. aureus to colonize implanted prosthetic devices. The data revealed that S. aureus isolates from the same clonal complex (CC) contain a specific pattern of SNPs in the regulatory region of genes encoding surface adhesins. As a consequence, each clonal lineage shows a specific profile of surface proteins expression. Co-infection experiments with representative isolates of the most prevalent CCs demonstrated that some lineages have a higher capacity to colonize implanted catheters in a murine infection model, which correlated with a greater ability to form a biofilm on coated surfaces with plasma proteins. Together, results indicate that differences in the expression level of surface adhesins may modulate the propensity of S. aureus strains to cause PJI. Given the high conservation of surface proteins among staphylococci, our work lays the framework for investigating how diversification at intergenic regulatory regions affects the capacity of S. aureus to colonize the surface of medical implants.	en
dc.description.sponsorship	This work was financially supported by the Spanish Ministry of Science and Innovation grant PID2020-113494RB-I00 to I.L. (Agencia Española de Investigación/Fondo Europeo de Desarrollo Regional, European Union), the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 754412 [MoRE2020 - Region Väs-tra Götaland], and the IngaBritt and Arne Lundberg Foundation (LU2021-0048). L.M.L was supported by the European Union's H2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 801586 (IberusTalent).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/msword	en
dc.identifier.citation	Morales-Laverde, L., Trobos, M., Echeverz, M., Solano, C., & Lasa, I. (2022). Functional analysis of intergenic regulatory regions of genes encoding surface adhesins in Staphylococcus aureus isolates from periprosthetic joint infections. Biofilm, 4, 100093. https://doi.org/10.1016/j.bioflm.2022.100093	en
dc.identifier.doi	10.1016/j.bioflm.2022.100093
dc.identifier.issn	2590-2075
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44821
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Biofilm 4 (2022) 100093	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113494RB-I00/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/754412/
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586/
dc.relation.publisherversion	https://doi.org/10.1016/j.bioflm.2022.100093
dc.rights	© 2022 The Authors. This is an open access article under the CC BY-NC-ND license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Periprosthetic joint infection	en
dc.subject	LPXTG proteins	en
dc.subject	SNPs	en
dc.subject	Intergenic regions	en
dc.subject	Staphylococcus aureus	en
dc.subject	Biofilm	en
dc.title	Functional analysis of intergenic regulatory regions of genes encoding surface adhesins in Staphylococcus aureus isolates from periprosthetic joint infections	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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