Chemically defined conditions mediate an efficient induction of dental pulp pluripotent-like stem cells into hepatocyte-like cells

Gil-Recio, Carlos; Montori, Sheyla; Al Demour, Saddam; Ababneh, Mera A.; Ferrés-Padró, Eduard; Marti, Carles; Ferrés-Amat, Elvira; Barajas Vélez, Miguel Ángel; Al Madhoun, Ashraf; Atari, Maher

Publication:
Chemically defined conditions mediate an efficient induction of dental pulp pluripotent-like stem cells into hepatocyte-like cells

dc.contributor.author	Gil-Recio, Carlos	es_ES
dc.contributor.author	Montori, Sheyla	es_ES
dc.contributor.author	Al Demour, Saddam	es_ES
dc.contributor.author	Ababneh, Mera A.	es_ES
dc.contributor.author	Ferrés-Padró, Eduard	es_ES
dc.contributor.author	Marti, Carles	es_ES
dc.contributor.author	Ferrés-Amat, Elvira	es_ES
dc.contributor.author	Barajas Vélez, Miguel Ángel
dc.contributor.author	Al Madhoun, Ashraf	es_ES
dc.contributor.author	Atari, Maher	es_ES
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.date.accessioned	2022-04-07T07:47:48Z
dc.date.available	2022-04-07T07:47:48Z
dc.date.issued	2021
dc.description.abstract	Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to generate functional hepatocyte-like cells in vitro. Cells were differentiated on a collagen scaffold in serum-free media supplemented with growth factors and cytokines to recapitulate liver development. At day 5, the differentiated DPPSC cells expressed the endodermal markers FOXA1 and FOXA2. Then, the cells were derived into the hepatic lineage generating hepatocyte-like cells. In addition to the associated morphological changes, the cells expressed the hepatic genes HNF6 and AFP. The terminally differentiated hepatocyte-like cells expressed the liver functional proteins albumin and CYP3A4. In this study, we report an efficient serum-free protocol to differentiate DPPSCs into functional hepatocyte-like cells. Our approach promotes the use of DPPSCs as a new source of adult stem cells for prospective use in liver regenerative medicine.	en
dc.description.sponsorship	This study was funded by the Universitat Internacional de Catalunya (UIC), the Agència de Gestió d’Ajuts Universitaris i de Recerca, Generalitat de Catalunya project number (SGR 1060 for MA), the Kuwait Foundation for the Advancement of Sciences (KFAS), and the Dasman Diabetes Institute under project number (RA-2013-009 for AAM). CGR, EMS, and RNT were funded by the predoctoral grant Junior Faculty award from the Obra Social, 'la Caixa' Foundation, and UIC.	en
dc.format.extent	14 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1155/2021/5212852
dc.identifier.issn	1687-9678
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/42684
dc.language.iso	eng	en
dc.publisher	Hindawi	en
dc.relation.ispartof	Stem Cells International, Volume 2021, Article ID 5212852, 14 pages	en
dc.relation.publisherversion	https://doi.org/10.1155/2021/5212852
dc.rights	© 2021 Carlos Gil-Recio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.	es_ES
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Dental pulp pluripotent-like stem cells (DPPSCs)	en
dc.subject	Hepatocyte-like cells	en
dc.title	Chemically defined conditions mediate an efficient induction of dental pulp pluripotent-like stem cells into hepatocyte-like cells	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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