Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles

Simple item page
dc.contributor.authorGámez Arcas, Samuel
dc.contributor.authorMuñoz, Francisco José
dc.contributor.authorRicarte Bermejo, Adriana
dc.contributor.authorSánchez López, Ángela María
dc.contributor.authorBaslam, Marouane
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorDiego, Nuria de
dc.contributor.authorDolezal, Karel
dc.contributor.authorNovák, Ondrej
dc.contributor.authorLeal-López, Jesús
dc.contributor.authorLeón Morcillo, Rafael Jorge
dc.contributor.authorCastillo, Araceli G.
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.date.accessioned2023-03-28T14:16:08Z
dc.date.available2023-03-28T14:16:08Z
dc.date.issued2022
dc.date.updated2023-03-28T14:09:23Z
dc.description.abstractIn Arabidopsis (Arabidopsis thaliana), the plastidial isoform of phosphoglucose isomerase (PGI1) mediates photosynthesis, metabolism, and development, probably due to its involvement in the synthesis of isoprenoid-derived signals in vascular tissues. Microbial volatile compounds (VCs) with molecular masses of 545 Da promote photosynthesis, growth, and starch overaccumulation in leaves through PGI1-independent mechanisms. Exposure to these compounds in leaves enhances the levels of GLUCOSE-6-PHOSPHATE/PHOSPHATE TRANSLOCATOR2 (GPT2) transcripts. We hypothesized that the PGI1-independent response to microbial volatile emissions involves GPT2 action. To test this hypothesis, we characterized the responses of wild-type (WT), GPT2-null gpt2-1, PGI1-null pgi1-2, and pgi1-2gpt2-1 plants to small fungal VCs. In addition, we characterized the responses of pgi1-2gpt2-1 plants expressing GPT2 under the control of a vascular tissue- and root tip-specific promoter to small fungal VCs. Fungal VCs promoted increases in growth, starch content, and photosynthesis in WT and gpt2-1 plants. These changes were substantially weaker in VC-exposed pgi1-2gpt2-1 plants but reverted to WT levels with vascular and root tip-specific GPT2 expression. Proteomic analyses did not detect enhanced levels of GPT2 protein in VC-exposed leaves and showed that knocking out GPT2 reduced the expression of photosynthesis-related proteins in pgi1-2 plants. Histochemical analyses of GUS activity in plants expressing GPT2-GUS under the control of the GPT2 promoter showed that GPT2 is mainly expressed in root tips and vascular tissues around hydathodes. Overall, the data indicated that the PGI1-independent response to microbial VCs involves resetting of the photosynthesis-related proteome in leaves through long-distance GPT2 action.en
dc.description.sponsorshipThis work was supported by the Ministerio de Ciencia e Innovación (MCIN) and Agencia Estatal de Investigación (AEI)/10.13039/501100011033/ (grants BIO2016-78747-P, PID2019-104685GB-100 and PID2019-107657RB-C22) and the Ministry of Education, Youth and Sport of the Czech Republic and the European Regional Development Fund (ERDF) project entitled “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/ 0000827).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.citationGámez-Arcas, S., Muñoz, F. J., Ricarte-Bermejo, A., Sánchez-López, Á. M., Baslam, M., Baroja-Fernández, E., Bahaji, A., Almagro, G., De Diego, N., Dolezal K., Novák, O., Leal-López, J., Morcillo, R. J. L., Castillo, A. G., Pozueta-Romero, J. (2022) Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles. Plant Physiology, 190(4), 2137-2154. https://doi.org/10.1093/plphys/kiac433.en
dc.identifier.doi10.1093/plphys/kiac433
dc.identifier.issn0032-0889
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/44971
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofPlant Physiology, 190(4), (2022), 2137–2154en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI//BIO2016-78747-P/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI//PID2019-104685GB/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107657RB-C22/ES/
dc.relation.publisherversionhttps://doi.org/10.1093/plphys/kiac433
dc.rights© The Author(s) 2022. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMicrobial volatile compounds (VCs)en
dc.subjectGPT2en
dc.titleGlucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatilesen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication041a1a7f-8b33-491a-8a52-015063f9b1ae
relation.isAuthorOfPublication2d9a4a11-cb33-4712-8860-74cd203cd53d
relation.isAuthorOfPublication44f8348d-8a3c-4f89-bbaa-8303920682c3
relation.isAuthorOfPublication0a533d7e-81fb-4439-ad05-2927e96fa923
relation.isAuthorOfPublication98c9658b-9c03-41bf-9966-776fa23ec157
relation.isAuthorOfPublicationbcd76277-79d9-4cf7-b6bd-f172dabe984b
relation.isAuthorOfPublication80c8bd69-4127-4474-a679-3b10747cae42
relation.isAuthorOfPublication.latestForDiscovery041a1a7f-8b33-491a-8a52-015063f9b1ae

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Gamez_Glucose6P.pdf
Size:
2.27 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
Gamez_Glucose6P_MatCompl.zip
Size:
1.14 MB
Format:
ZIP
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.78 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista DABA - ABES Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak