Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: implications for the production and biosecurity of baculovirus insecticides

Carballo Palos, Arkaitz; Murillo Pérez, Rosa; Jakubowska, Agata; Herrero, Salvador; Williams, Trevor; Caballero Murillo, Primitivo

Publication:
Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: implications for the production and biosecurity of baculovirus insecticides

dc.contributor.author	Carballo Palos, Arkaitz
dc.contributor.author	Murillo Pérez, Rosa
dc.contributor.author	Jakubowska, Agata
dc.contributor.author	Herrero, Salvador
dc.contributor.author	Williams, Trevor
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-09-06T12:20:20Z
dc.date.available	2018-09-06T12:20:20Z
dc.date.issued	2017
dc.description.abstract	Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection on the insecticidal characteristics of SeMNPV occlusion bodies (OBs). Overall, iflavirus coinoculation consistently reduced median lethal concentrations (LC50) for SeMNPV OBs compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. A reduction of the weight gain (27%) associated with iflavirus infection resulted in a 30% reduction in total OB production per larva. Adult survivors of SeMNPV OB inoculation were examined for covert infection. SeMNPV DNA was found to be present at a high prevalence in all SeIV-1 and SeIV-2 coinfection treatments. Interestingly, co-inoculation of SeMNPV with SeIV-2 alone or in mixtures with SeIV-1 resulted in a significant increase in the SeMNPV load of sublethally infected adults, suggesting a role for SeIV-2 in vertical transmission or reactivation of sublethal SeMNPV infections. In conclusion, iflaviruses are not desirable in insect colonies used for large scale baculovirus production, as they may result in diminished larval growth, reduced OB production and, depending on their host-range, potential risks to non-target Lepidoptera.	en
dc.description.sponsorship	This study was funded by the Spanish Ministerio de Ciencia e Innovación, project number AGL2011-30352-C02-01. AC received a student scholarship from the Ministerio de Economía y Competitividad.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0177301
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30549
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 12(5):e0177301	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30352-C02-01/ES/	en
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0177301
dc.rights	© 2017 Carballo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	SeMNPV occlusion bodies	en
dc.subject	Iflavirus co-infection	en
dc.subject	Biological insecticides	en
dc.title	Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: implications for the production and biosecurity of baculovirus insecticides	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
relation.isAuthorOfPublication	e283ed01-c91a-4878-b413-a4251e792835
relation.isAuthorOfPublication	46f59760-02e7-4f4d-bfb2-e505b2875f48
relation.isAuthorOfPublication	0b5a3470-9d73-4ac7-a923-c4f6079df358
relation.isAuthorOfPublication	ecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery	e283ed01-c91a-4878-b413-a4251e792835