Fitness cost evolution of natural plasmids of staphylococcus aureus

Dorado Morales, Pedro; Garcillán-Barcia, María Pilar; Lasa Uzcudun, Íñigo; Solano Goñi, Cristina

Fitness cost evolution of natural plasmids of staphylococcus aureus

dc.contributor.author	Dorado Morales, Pedro
dc.contributor.author	Garcillán-Barcia, María Pilar
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.author	Solano Goñi, Cristina
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.date.accessioned	2021-12-09T12:25:20Z
dc.date.available	2021-12-09T12:25:20Z
dc.date.issued	2021
dc.description.abstract	Plasmids have largely contributed to the spread of antimicrobial resistance genes among Staphylococcus strains. Knowledge about the fitness cost that plasmids confer on clinical staphylococcal isolates and the coevolutionary dynamics that drive plasmid maintenance is still scarce. In this study, we aimed to analyze the initial fitness cost of plasmids in the bacterial pathogen Staphylococcus aureus and the plasmid-host adaptations that occur over time. For that, we first designed a CRISPR (clustered regularly interspaced palindromic repeats)-based tool that enables the removal of native S. aureus plasmids and then transferred three different plasmids isolated from clinical S. aureus strains to the same-background clinical cured strain. One of the plasmids, pUR2940, obtained from a livestock-associated methicillin-resistant S. aureus (LA-MRSA) ST398 strain, imposed a significant fitness cost on both its native and the new host. Experimental evolution in a nonselective medium resulted in a high rate pUR2940 loss and selected for clones with an alleviated fitness cost in which compensatory adaptation occurred via deletion of a 12.8-kb plasmid fragment, contained between two ISSau10 insertion sequences and harboring several antimicrobial resistance genes. Overall, our results describe the relevance of plasmid-borne insertion sequences in plasmid rearrangement and maintenance and suggest the potential benefits of reducing the use of antibiotics both in animal and clinical settings for the loss of clinical multidrug resistance plasmids.	en
dc.description.sponsorship	This work was financially supported by the Spanish Ministry of Science, Innovationand Universities grants BIO2014-53530-R and BIO2017-83035-R (Agencia Española de Investigación/Fondo Europeo de Desarrollo Regional, European Union) to I.L. and C.S. P.D.-M. was supported by an F.P.I. (BES-2015-072859) contract from the Spanish Ministry of Science, Innovation and Universities.	en
dc.format.extent	18 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1128/mBio.03094-20
dc.identifier.issn	2150-7511 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/41218
dc.language.iso	eng	en
dc.publisher	American Society for Microbiology	en
dc.relation.ispartof	mBio 12:e03094-20	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2014-53530-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83035-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BES-2015-072859/ES/
dc.relation.publisherversion	https://doi.org/10.1128/mBio.03094-20
dc.rights	© 2021 Dorado-Morales et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Plasmid	en
dc.subject	Fitness cost	en
dc.subject	Evolution	en
dc.subject	Staphylococcus aureus	en
dc.subject	CRISPR	en
dc.subject	Insertion sequences	en
dc.subject	Antibiotic resistance	en
dc.title	Fitness cost evolution of natural plasmids of staphylococcus aureus	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	886fbc8e-fb8b-46b9-aa6f-9b42e324ac85
relation.isAuthorOfPublication	c654d104-1ae2-41cf-9215-4b4bed3e5ea6
relation.isAuthorOfPublication	b6a392a0-c0e5-4c6f-9086-6fa4291d0856
relation.isAuthorOfPublication.latestForDiscovery	886fbc8e-fb8b-46b9-aa6f-9b42e324ac85