Pseudomonas savastanoi pv. mandevillae pv. nov., a clonal pathogen causing an emerging, devastating disease of the ornamental plant Mandevilla spp.

Caballo Ponce, Eloy; Pintado, Adrián; Moreno Pérez, Alba; Murillo Martínez, Jesús; Smalla, Kornelia; Ramos, Cayo

Publication:
Pseudomonas savastanoi pv. mandevillae pv. nov., a clonal pathogen causing an emerging, devastating disease of the ornamental plant Mandevilla spp.

dc.contributor.author	Caballo Ponce, Eloy
dc.contributor.author	Pintado, Adrián
dc.contributor.author	Moreno Pérez, Alba
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.author	Smalla, Kornelia
dc.contributor.author	Ramos, Cayo
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2021-04-26T19:13:52Z
dc.date.issued	2021
dc.description.abstract	Commercial production of the ornamental plant dipladenia (Mandevilla spp.) is threatened by dipladenia leaf and stem spot disease, caused by the bacterium Pseudomonas savastanoi. P. savastanoi includes four pathovars of woody hosts differentiated by a characteristic host range in olive, oleander, ash and broom plants. However, isolates from dipladenia have not been ascribed to any particular lineage or P. savastanoi pathovar. Here we report that isolates from dipladenia represent a distinct, clonal lineage. First, dipladenia isolates display very similar plasmid profiles, including a plasmid encoding the iaaM gene for biosynthesis of indole-3-acetic acid. Second, multilocus sequence analysis and core-genome single-nucleotide-polymorphisms phylogenies showed a monophyletic origin for dipladenia isolates, which cluster with isolates from oleander (pathovar nerii) in a distinct clade well separated from other P. savastanoi strains. Metabolic profiling and cross-pathogenicity tests in olive, oleander, ash, broom and dipladenia clearly distinguished dipladenia isolates from the four P. savastanoi pathovars. Comparative genomics of the draft genome sequence of the dipladenia strain Ph3 with the other four pathovars showed that Ph3 encodes very few strain-specific genes, and a similar set of virulence genes to pv. nerii, including its repertoire of type III secretion system effectors. However, hierarchical clustering based on the catalogue of effectors and their allelic variants clearly separated Ph3 from pv. nerii strains. Based on their distinctive pathogenicity profile, we propose a de novo pathovar for P. savastanoi isolates from dipladenia, P. savastanoi pv. mandevillae pv. nov., for which strain Ph3 (CFBP 8832PT) has been designated as the pathotype strain.	en
dc.description.sponsorship	E.C.P, A.M.P, A.P, C.R and J.M were supported by grants FPI/BES20 2012-052398, FPI/BES-2015-074847, FPU14/05551, AGL2017-82492-C2-1-R and AGL2017-82492-C2-2-R, respectively, from Ministerio de Ciencia, Innovación y Universidades (Spain), cofinanced by the Fondo Europeo de Desarrollo Regional (FEDER).	en
dc.format.extent	54 p.
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Caballo-Ponce, E., Pintado, A., Moreno-Pérez, A., Murillo, J., Smalla, K., & Ramos, C. (2021). Pseudomonas savastanoi pv. Mandevillae pv. Nov. , A clonal pathogen causing an emerging, devastating disease of the ornamental plant mandevilla spp. Phytopathology®, PHYTO-11-20-0526-R. https://doi.org/10.1094/PHYTO-11-20-0526-R	en
dc.identifier.doi	10.1094/PHYTO-11-20-0526-R
dc.identifier.issn	0031-949X (Print)
dc.identifier.issn	1943-7684 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/39609
dc.language.iso	eng	en
dc.publisher	APS Publications	en
dc.relation.ispartof	Phytopathology®, PHYTO-11-20-0526-R	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R/ES/	en
dc.relation.publisherversion	https://doi.org/10.1094/PHYTO-11-20-0526-R
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.subject	Pseudomonas syringae complex	en
dc.subject	Pseudomonas amygdali	en
dc.subject	Host range	en
dc.subject	Knot disease	en
dc.subject	New pathovar	en
dc.subject	Phytohormones	en
dc.subject	Type III secretion system	en
dc.subject	Type III effectors	en
dc.subject	Comparative genomics	en
dc.subject	Phylogenomics	en
dc.subject	Metabolic profile	en
dc.title	Pseudomonas savastanoi pv. mandevillae pv. nov., a clonal pathogen causing an emerging, devastating disease of the ornamental plant Mandevilla spp.	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
relation.isAuthorOfPublication	3f350c70-2e68-4b9b-95d6-26e9cc1540a0
relation.isAuthorOfPublication.latestForDiscovery	3f350c70-2e68-4b9b-95d6-26e9cc1540a0