Publication: Generation of variability in Chrysodeixis includens nucleopolyhedrovirus (ChinNPV): the role of a single variant
| dc.contributor.author | Aguirre Sánchez, Eduardo | |
| dc.contributor.author | Beperet Arive, Inés | |
| dc.contributor.author | Williams, Trevor | |
| dc.contributor.author | Caballero Murillo, Primitivo | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.date.accessioned | 2021-09-28T07:58:38Z | |
| dc.date.available | 2021-09-28T07:58:38Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses to evolve with their host and with changes in their environment. We examined the role of one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPVMex1) contained two genetic variants that dominated over other variants in individual larvae that consumed high (ChinNPV-K) and low (ChinNPV-E) concentrations of inoculum. Studies on the ChinNPV-K variant indicated that it was capable of generating novel variation in a concentrationdependent manner. In cell culture, cells inoculated with high concentrations of ChinNPV-K produced OBs with the ChinNPV-K REN profile, whereas a high diversity of ChinNPV variants was recovered following plaque purification of low concentrations of ChinNPV-K virion inoculum. Interestingly, the ChinNPV-K variant could not be recovered from plaques derived from low concentration inocula originating from budded virions or occlusion-derived virions of ChinNPV-K. Genome sequencing revealed marked differences between ChinNPV-K and ChinNPV-E, with high variation in the ChinNPV-K genome, mostly due to single nucleotide polymorphisms. We conclude that ChinNPV-K is an unstable genetic variant that is responsible for generating much of the detected variability in the natural ChinNPV isolates used in this study. | en |
| dc.description.sponsorship | This research was funded by the Ministerio de Economía y Competitividad; grant number AGL2017-83498-C2-1-R. E.A. received an UPNA studentship. I.B. received a Torres-Quevedo grant and was funded by Bioinsectis SL during the preparation of this manuscript. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.3390/v13101895 | |
| dc.identifier.issn | 1999-4915 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/40586 | |
| dc.language.iso | eng | en |
| dc.publisher | MDPI | en |
| dc.relation.ispartof | Viruses 2021, 13, 1895 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83498-C2-1-R/ES/ | en |
| dc.relation.publisherversion | https://doi.org/10.3390/v13101895 | |
| dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license | en |
| dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | ChinNPV | en |
| dc.subject | Variability | en |
| dc.subject | Prevalence | en |
| dc.subject | SNPs | en |
| dc.subject | Concentration | en |
| dc.subject | Chrysodeixis includens | en |
| dc.title | Generation of variability in Chrysodeixis includens nucleopolyhedrovirus (ChinNPV): the role of a single variant | en |
| dc.type | Artículo / Artikulua | es |
| dc.type | info:eu-repo/semantics/article | en |
| dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
| dc.type.version | info:eu-repo/semantics/publishedVersion | en |
| dspace.entity.type | Publication | |
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