Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/JCM.01302-13
Resumen
Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there
are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of
the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification
of HPV DNA ...
[++]
Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there
are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of
the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification
of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and
urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types
(HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the
cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples,
with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly
with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous
cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics
for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the
detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control
programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical,
are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples. [--]
Materias
Human papillomavirus (HPV),
HIV,
PCR-based moleculat tests,
Urine samples
Editor
American Society for Microbiology
Publicado en
Journal of Clinical Microbiology, November 2013 vol. 51 no. 11 3688-3693
Notas
Incluye un fichero de datos
Departamento
Universidad Pública de Navarra. Departamento de Estadística e Investigación Operativa /
Nafarroako Unibertsitate Publikoa. Estatistika eta Ikerketa Operatiboa Saila
Versión del editor
Entidades Financiadoras
The authors are grateful to the Basque Cooperation Agency for
Development and the Spanish Agency for International Development Cooperation
(AECID) for supporting and financing this project.