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dc.creatorMuñoz, Marinaes_ES
dc.creatorCamargo, Milenaes_ES
dc.creatorSoto de León, Saraes_ES
dc.creatorSánchez, Ricardoes_ES
dc.creatorPineda Peña, Andreaes_ES
dc.creatorPérez Prados, Antonioes_ES
dc.creatorPatarroyo, Manuel Elkines_ES
dc.creatorPatarroyo, Manuel Alfonsoes_ES
dc.identifier.issn0095-1137 (Print)
dc.identifier.issn1098-660X (Electronic)
dc.descriptionIncluye un fichero de datoses_ES
dc.description.abstractHuman papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.en
dc.description.sponsorshipThe authors are grateful to the Basque Cooperation Agency for Development and the Spanish Agency for International Development Cooperation (AECID) for supporting and financing this project.en
dc.format.extent6 p.
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Clinical Microbiology, November 2013 vol. 51 no. 11 3688-3693en
dc.rights© 2013 American Society for Microbiology. All Rights Reserved.en
dc.subjectHuman papillomavirus (HPV)en
dc.subjectPCR-based moleculat testsen
dc.subjectUrine samplesen
dc.titleClassical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected womenen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentEstadística e Investigación Operativaes_ES
dc.contributor.departmentEstatistika eta Ikerketa Operatiboaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión publicada / Argitaratu den bertsioaes

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