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dc.creatorParras-Jurado, Anaes_ES
dc.creatorMuñoz Labiano, Deliaes_ES
dc.creatorBeperet Arive, Inéses_ES
dc.creatorWilliams, Trevores_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.date.accessioned2023-10-20T14:42:07Z
dc.date.available2023-10-20T14:42:07Z
dc.date.issued2023
dc.identifier.citationParras-Jurado, A., Muñoz, D., Beperet, I., Williams, T., Caballero, P. (2023) Insecticidal traits of variants in a genotypically diverse natural isolate of anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV). Viruses, 15(7), 1-13. https://doi.org/10.3390/v15071526.en
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/2454/46602
dc.description.abstractOutbreaks of Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Erebidae), a major pest of soybean, can be controlled below economic thresholds with methods that do not involve the application of synthetic insecticides. Formulations based on natural isolates of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) (Baculoviridae: Alphabaculovirus) played a significant role in integrated pest management programs in the early 2000s, but a new generation of chemical insecticides and transgenic soybean have displaced AgMNPV-based products over the past decade. However, the marked genotypic variability present among and within alphabaculovirus isolates suggests that highly insecticidal genotypic variants can be isolated and used to reduce virus production costs or overcome isolate-dependent host resistance. This study aimed to select novel variants of AgMNPV with suitable insecticidal traits that could complement the existing AgMNPV active ingredients. Three distinct AgMNPV isolates were compared using their restriction endonuclease profile and in terms of their occlusion body (OB) pathogenicity. One isolate was selected (AgABB51) from which eighteen genotypic variants were plaque purified and characterized in terms of their insecticidal properties. The five most pathogenic variants varied in OB pathogenicity, although none of them was faster-killing or had higher OB production characteristics than the wild-type isolate. We conclude that the AgABB51 wild-type isolates appear to be genotypically structured for fast speed of kill and high OB production, both of which would favor horizontal transmission. Interactions among the component variants are likely to influence this insecticidal phenotype.en
dc.description.sponsorshipThis research was funded by the Ministerio de Ciencia e Innovacion, grant number AGL2017-83498-C2-1-R. A.P.-J. received a predoctoral grant PRE2018-086829 from the Agencia Estatal de Investigación (Ministerio de Ciencia e Innovación) of the Spanish Government.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofViruses 2023, 15(7), 1526en
dc.rights© 2023 by the authors. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBaculoviridaeen
dc.subjectBioinsecticideen
dc.subjectGenotypic varianten
dc.subjectOcclusion body productionen
dc.subjectPathogenicityen
dc.subjectVirulenceen
dc.titleInsecticidal traits of variants in a genotypically diverse natural isolate of anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)en
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.date.updated2023-10-20T14:13:09Z
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.3390/v15071526
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83498-C2-1-R/ES/en
dc.relation.publisherversionhttps://doi.org/10.3390/v15071526
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2023 by the authors. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as © 2023 by the authors. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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