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dc.creatorBardají Goikoetxea, Leirees_ES
dc.creatorAñorga García, Maitees_ES
dc.creatorJackson, Robert W.es_ES
dc.creatorMartínez Bilbao, Alejandroes_ES
dc.creatorYanguas Casas, Nataliaes_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.date.accessioned2014-05-16T08:54:40Z
dc.date.available2014-05-16T08:54:40Z
dc.date.issued2011
dc.identifier.issn1932-6203
dc.identifier.other553
dc.identifier.urihttps://hdl.handle.net/2454/10547
dc.descriptionUPNa. Departamento de Producción Agraria. Laboratorio de Patología Vegetales_ES
dc.description.abstractMobile genetic elements are widespread in Pseudomonas syringae, and often associate with virulence genes. Genome reannotation of the model bean pathogen P. syringae pv. phaseolicola 1448A identified seventeen types of insertion sequences and two miniature inverted-repeat transposable elements (MITEs) with a biased distribution, representing 2.8% of the chromosome, 25.8% of the 132-kb virulence plasmid and 2.7% of the 52-kb plasmid. Employing an entrapment vector containing sacB, we estimated that transposition frequency oscillated between 2.6 x 10(-5) and 1.1 x 10(-6), depending on the clone, although it was stable for each clone after consecutive transfers in culture media. Transposition frequency was similar for bacteria grown in rich or minimal media, and from cells recovered from compatible and incompatible plant hosts, indicating that growth conditions do not influence transposition in strain 1448A. Most of the entrapped insertions contained a full-length IS801 element, with the remaining insertions corresponding to sequences smaller than any transposable element identified in strain 1448A, and collectively identified as miniature sequences. From these, fragments of 229, 360 and 679-nt of the right end of IS801 ended in a consensus tetranucleotide and likely resulted from one-ended transposition of IS801. An average 0.7% of the insertions analyzed consisted of IS801 carrying a fragment of variable size from gene PSPPH_0008/PSPPH_0017, showing that IS801 can mobilize DNA in vivo. Retrospective analysis of complete plasmids and genomes of P. syringae suggests, however, that most fragments of IS801 are likely the result of reorganizations rather than one-ended transpositions, and that this element might preferentially contribute to genome flexibility by generating homologous regions of recombination. A further miniature sequence previously found to affect host range specificity and virulence, designated MITEPsy1 (100-nt), represented an average 2.4% of the total number of insertions entrapped in sacB, demonstrating for the first time the mobilization of a MITE in bacteria.en
dc.description.sponsorshipThis work was funded with the Spanish Plan Nacional I+D+I grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e Innovación; http://www.micinn.es/) co financed by FEDER.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 2011, 6 (10): e25773es
dc.rights© 2011 Bardaji et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectArtemis comparison toolen
dc.subjectFlank avirulence genesen
dc.subjectInsertion sequencesen
dc.subjectTomato DC3000en
dc.subjectPathovaren
dc.titleMiniature transposable sequences are frequently mobilized in the bacterial plant pathogen Pseudomonas syringae pv. phaseolicolaen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.affiliationUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.affiliationNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1371/journal.pone.0025773
dc.relation.publisherversionhttps://dx.doi.org/10.1371/journal.pone.0025773
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2011 Bardaji et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2011 Bardaji et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.