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dc.creatorBardají Goikoetxea, Leirees_ES
dc.creatorAñorga García, Maitees_ES
dc.creatorRuiz Masó, José A.es_ES
dc.creatorSolar, Gloria deles_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.date.accessioned2017-02-14T13:15:31Z
dc.date.available2017-02-14T13:15:31Z
dc.date.issued2017
dc.identifier.issn1664-302X (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/23654
dc.descriptionIncluye ficheros de datoses_ES
dc.description.abstractPlasmids are a main factor for the evolution of bacteria through horizontal gene exchange, including the dissemination of pathogenicity genes, resistance to antibiotics and degradation of pollutants. Their capacity to duplicate is dependent on their replication determinants (replicon), which also define their bacterial host range and the inability to coexist with related replicons. We characterize a second replicon from the virulence plasmid pPsv48C, from Pseudomonas syringae pv. savastanoi, which appears to be a natural chimera between the gene encoding a newly described replication protein and a putative replication control region present in the widespread family of PFP virulence plasmids. We present extensive evidence of this type of chimerism in structurally similar replicons from species of Pseudomonas, including environmental bacteria as well as plant, animal and human pathogens. We establish that these replicons consist of two functional modules corresponding to putative control (REx-C module) and replication (REx-R module) regions. These modules are functionally separable, do not show specificity for each other, and are dynamically exchanged among replicons of four distinct plasmid families. Only the REx-C module displays strong incompatibility, which is overcome by a few nucleotide changes clustered in a stem-and-loop structure of a putative antisense RNA. Additionally, a REx-C module from pPsv48C conferred replication ability to a non-replicative chromosomal DNA region containing features associated to replicons. Thus, the organization of plasmid replicons as independent and exchangeable functional modules is likely facilitating rapid replicon evolution, fostering their diversification and survival, besides allowing the potential co-option of appropriate genes into novel replicons and the artificial construction of new replicon specificities.en
dc.description.sponsorshipThis work was funded by the Spanish Plan Nacional I+D+i grant AGL2014-53242-C2-2-R, from the Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER). M.A. was supported by an FPI fellowship (reference BES-2012-054016, Ministerio de Ciencia e Innovación/Ministerio de Economía y Competitividad, Spain).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Microbiology 8: 190en
dc.rights© 2017 Bardaji, Añorga, Ruiz-Masó, del Solar and Murillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectControl and replication modulesen
dc.subjectChimeric repliconsen
dc.subjectGene co-optionen
dc.subjectRep proteinsen
dc.subjectOrigin of replicationen
dc.subjectPlasmid incompatibilityen
dc.subjectSwapping of functional modulesen
dc.subjectVirulence plasmidsen
dc.titlePlasmid replicons from Pseudomonas are natural chimeras of functional, exchangeable modulesen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.3389/fmicb.2017.00190
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/AGL2014-53242en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-30343en
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2017.00190
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2017 Bardaji, Añorga, Ruiz-Masó, del Solar and Murillo. This is an
open-access article distributed under the terms of the Creative Commons Attribution
License (CC BY). The use, distribution or reproduction in other forums is permitted,
provided the original author(s) or licensor are credited and that the original
publication in this journal is cited, in accordance with accepted academic practice.
No use, distribution or reproduction is permitted which does not comply with these
terms.
Except where otherwise noted, this item's license is described as © 2017 Bardaji, Añorga, Ruiz-Masó, del Solar and Murillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.