Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways in Medicago truncatula roots and nodules
Larrainzar Rodríguez, Estíbaliz
Molenaar, Johanna A.
Gil Quintana, Erena
Limami, Anis M.
Arrese-Igor Sánchez, César
González García, Esther
Acceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess
Artículo / Artikuluainfo:eu-repo/semantics/article
Versión aceptada / Onetsi den bertsioainfo:eu-repo/semantics/acceptedVersion
Symbiotic nitrogen fixation is one of the first physiological processes inhibited in legume plants under water-deficit conditions. Despite the progress made in the last decades, the molecular mechanisms behind this regulation are not fully understood yet. Recent proteomic work carried out in the model legume Medicago truncatula provided the first indications of a possible involvement of nod ... [++]
Symbiotic nitrogen fixation is one of the first physiological processes inhibited in legume plants under water-deficit conditions. Despite the progress made in the last decades, the molecular mechanisms behind this regulation are not fully understood yet. Recent proteomic work carried out in the model legume Medicago truncatula provided the first indications of a possible involvement of nodule methionine (Met) biosynthesis and related pathways in response to waterdeficit conditions. To better understand this involvement, the drought-induced changes in expression and content of enzymes involved in the biosynthesis of Met, S-adenosyl-Lmethionine (SAM) and ethylene in M. truncatula root and nodules were analyzed using targeted approaches. Nitrogenfixing plants were subjected to a progressive water deficit and a subsequent recovery period. Besides the physiological characterization of the plants,the content of total sulphur,sulphate and main S-containing metabolites was measured. Results presented here show that S availability is not a limiting factor in the drought-induced decline of nitrogen fixation rates in M. truncatula plants and provide evidences for a downregulation of the Met and ethylene biosynthesis pathways in roots and nodules in response to water-deficit conditions. [--]
Symbiosis, Proteome, Sulfur metabolism.
Plant, Cell and Environment 37: 2051-2063
Incluye 3 ficheros de datos
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural / Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Versión del editor
This work was supported by the Spanish Ministry of Economy and Competitiveness (AGL 2011–23738 and AGL 2011–30386-C02-01).E.L. is a recipient of a Marie Curie International Outgoing Fellowship for Career Development (PIOF-GA-2009–253141). E.G-Q. received a PhD fellowship from the Public University of Navarre (735/2008).