Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways in Medicago truncatula roots and nodules
Fecha
2014Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Identificador del proyecto
Impacto
|
10.1111/pce.12285
Resumen
Symbiotic nitrogen fixation is one of the first physiological
processes inhibited in legume plants under water-deficit conditions.
Despite the progress made in the last decades, the
molecular mechanisms behind this regulation are not fully
understood yet. Recent proteomic work carried out in the
model legume Medicago truncatula provided the first indications
of a possible involvement of nod ...
[++]
Symbiotic nitrogen fixation is one of the first physiological
processes inhibited in legume plants under water-deficit conditions.
Despite the progress made in the last decades, the
molecular mechanisms behind this regulation are not fully
understood yet. Recent proteomic work carried out in the
model legume Medicago truncatula provided the first indications
of a possible involvement of nodule methionine (Met)
biosynthesis and related pathways in response to waterdeficit
conditions. To better understand this involvement, the
drought-induced changes in expression and content of
enzymes involved in the biosynthesis of Met, S-adenosyl-Lmethionine
(SAM) and ethylene in M. truncatula root and
nodules were analyzed using targeted approaches. Nitrogenfixing
plants were subjected to a progressive water deficit and
a subsequent recovery period. Besides the physiological characterization
of the plants,the content of total sulphur,sulphate
and main S-containing metabolites was measured. Results
presented here show that S availability is not a limiting factor
in the drought-induced decline of nitrogen fixation rates in
M. truncatula plants and provide evidences for a downregulation
of the Met and ethylene biosynthesis pathways in
roots and nodules in response to water-deficit conditions. [--]
Materias
Symbiosis,
Proteome,
Sulfur metabolism.
Editor
Wiley
Publicado en
Plant, Cell and Environment 37: 2051-2063
Notas
Incluye 3 ficheros de datos
Departamento
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural /
Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Versión del editor
Entidades Financiadoras
This work was supported by the Spanish Ministry of Economy and Competitiveness (AGL 2011–23738 and AGL 2011–30386-C02-01).E.L. is a recipient of a Marie Curie International Outgoing Fellowship for Career Development (PIOF-GA-2009–253141). E.G-Q. received a PhD fellowship from the Public University of Navarre (735/2008).