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Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes
dc.creator | Simón de Goñi, Oihane | es_ES |
dc.creator | Williams, Trevor | es_ES |
dc.creator | López Ferber, Miguel | es_ES |
dc.creator | Caballero Murillo, Primitivo | es_ES |
dc.date.accessioned | 2019-01-23T11:16:14Z | |
dc.date.available | 2019-01-23T11:16:14Z | |
dc.date.issued | 2004 | |
dc.identifier.issn | 0099-2240 (Print) | |
dc.identifier.issn | 1098-5336 (Electronic) | |
dc.identifier.uri | https://hdl.handle.net/2454/32070 | |
dc.description.abstract | A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant SfNIC-B was selected as the standard because its restriction profile corresponded to that of the wild-type isolate. Physical maps were generated for each of the variants. The differences between variants and the SfNIC-B standard were confined to the region between map units 9 and 32.5. This region included PstI-G, PstI-F, PstI-L, PstI-K and EcoRI-L fragments. Eight genotypes presented a deletion in their genome compared with SfNIC-B. Occlusion body-derived virions of SfNIC-C, -D and -G accounted for 41% of plaque-purified clones. These variants were not infectious per os but retained infectivity by injection into S. frugiperda larvae. Median 50% lethal concentration values for the other cloned genotypes were significantly higher than that of the wild type. The variants also differed in their speed of kill. Noninfectious variants SfNIC-C and -D lacked the pif and pif-2 genes. Infectivity was restored to these variants by plasmid rescue with a plasmid comprising both pif and pif-2. Transcription of an SfNIC-G gene was detected by reverse transcription-PCR in insects, but no fatal disease developed. Transcription was not detected in SfNIC-C or -D-inoculated larvae. We conclude that the SfNIC population presents high levels of genetic diversity, localized to a 17-kb region containing pif and pif-2, and that interactions among complete and deleted genotypic variants will likely influence the capacity of this virus to control insect pests. | en |
dc.format.extent | 10 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | American Society for Microbiology | en |
dc.relation.ispartof | Applied and Environmental Microbiology, vol. 70, nº 9, sept. 2004, p. 5579–5588 | en |
dc.rights | © 2004, American Society for Microbiology. All Rights Reserved. | en |
dc.subject | Spodoptera frugiperda Nucleopolyhedrovirus Population | en |
dc.title | Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Producción Agraria | es_ES |
dc.contributor.department | Nekazaritza Ekoizpena | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1128/aem.70.9.5579-5588.2004 | |
dc.relation.publisherversion | https://doi.org/10.1128/aem.70.9.5579-5588.2004 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |